Cat. # | Size | Qty. | Price | Inventory |
---|---|---|---|---|
5378S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 32 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human
Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences surrounding Pro5 of human Msx1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Msh homeobox 1 (Msx1) is a Muscle Segment Homeobox (Msh) gene family member that acts as a transcriptional repressor during embryonic development, playing an important role in limb pattern formation, craniofacial development, and tooth development (1-3). Msx1 is expressed in the mesenchyme of the developing nail bed (2) and in fetal hair follicles, epidermis and fibroblasts; reduced expression is seen in adult epithelial-derived tissues (4). Msx1 acts in concert with the Wnt1 network to establish the midbrain dopaminergic progenator domain, a region that gives rise to neurons that are critical for normal brain function and are the cells affected in Parkinson disease (5). Mutation in the corresponding Msx1 gene correlates with abnormal tooth development in patients diagnosed with Wolf-Hirschhorn syndrome (6). Other genetic changes in the Msx1 gene result in Witkop Syndrome ("tooth and nail syndrome") and cases of abnormal tooth development associated with non-syndromic orofacial clefting (2,7).
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