WDFY3 (F7P9J) Rabbit Monoclonal Antibody #96407

WD repeat and FYVE domain-containing 3 (WDFY3), also known as Alfy, is a large scaffolding protein with multiple domains implicated in membrane trafficking, including the FYVE zinc-finger, WD repeats with conserved LC-interacting regions (LIR) motifs, and a Pleckstrin homology (PH)-Beige and Chediak-Higashi (BEACH) domain (1). WDFY3 regulates autophagy via interactions with phosphatidyl-inositol 3-phosphate, ubiquitin-binding autophagy receptors like p62/SQSTM1, and selective proteins in the Atg8 family, particularly GABARAPs (1-3). WDFY3 contributes to multiple forms of selective autophagy, such as mitophagy and aggrephagy (4). WDFY3 is highly expressed in the developing central nervous system, and mutations in human WDFY3 have been shown to be a risk factor for intellectual and developmental disabilities (5-7). Likewise, loss of WDFY3 in mice results in neurodevelopment defects (8,9). WDFY3 is also required for the clearance of aggregated mutant huntingtin protein to confer resistance to Huntington’s disease (10). WDFY3 was also identified in a CRISPR screen as a regulator of efferocytosis, the phagocytic clearance of dying or dead cells (11). That study demonstrated that WDFY3 deficiency leads to impaired uptake of apoptotic cells and defects in degradation involving LC3-associated phagocytosis (LAP).