200 mM HEPES Buffer, pH 8.0 can be used as a 10X buffer stock for use in our PTMScan® protocols. Dilute the stock solution to 20mM final concentration using high-quality purified water, such as reverse osmosis deionized (RODI) purified or equivalent water.
Can be stored at 4°C for up to one month, long term storage should be at -20°C. Shelf life is 12 months at -20°C.
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a commonly used biological buffer due to superior physiological pH stability over a range of temperatures and conditions.
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