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6267
SignalSilence® Cofilin siRNA I
siRNA
siRNA

SignalSilence® Cofilin siRNA I #6267

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Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Cofilin siRNA I (+) or SignalSilence® Cofilin siRNA II #6268 (+), using Cofilin (D59) Antibody #3318 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Cofilin (D59) Antibody confirms silencing of cofilin expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Supporting Data

REACTIVITY H

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

製品概要

CST recommends transfection with 100 nM Cofilin siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

保存

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® Cofilin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit cofilin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

バックグラウンド

Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

  1. Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.
  2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
  3. Arber, S. et al. (1998) Nature 393, 805-809.
  4. Yang, N. et al. (1998) Nature 393, 809-812.
  5. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
  6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

Pathways & Proteins

Explore pathways + proteins related to this product.

研究用のみ。診断手順では使用しません。

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

To Purchase # 6267S
製品番号 サイズ 価格 在庫
6267S
300 µl  (3 nmol)

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.
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