心臓、脳などの組織サンプルや、マウス胚などでCUT&RUNを行うことはできますかか？

はい。We have shown that our CUT&RUN Assay Kit #86652 works with mouse liver, mouse brain, and mouse heart tissues with as little as 1mg of tissue for each CUT&RUN reaction. Fresh tissue samples work with histone modifications and high abundance DNA binding factors like CTCF. However, for optimal results with transcription factors, cofactors, and difficult tissue types, a light to medium fixation (0.1% formaldehyde, 2-10 min) should be performed and slightly more starting material (2.5mg-5mg) should be used. CUT&RUNアッセイで使用するまで、固定化された組織は最大6ヵ月、凍結保存できます。

Last updated: 2023 年 3 月 6 日