Is the SimpleChIP® Plus Sonication Chromatin IP Kit #56383 compatible with Covaris sonication/chromatin shearing?
はい。In our protocol, we recommend a probe-based sonicator because it is less expensive and widely used in many labs. However, you can use a Covaris water bath sonicator. You will still need to optimize the sonication program based on your DNA image, since you want to avoid over-sonicating when performing sonication-ChIP as this can damage chromatin quality/integrity. When looking at sonication results, chromatin DNA fragments should appear as a smear typically ranging between 200 to 1000 bp in size. We recommend you use the condition that requires the minimal sonication cycles, as long as the majority of DNA is less than 1000bp.
Last updated: 2023 年 3 月 13 日
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