Concanavalin Aビーズが、CUT&Tag実験で「凝集する」ことがあるのですが、注意事項はありますか？

Concanavalin A beads can clump together rather easily, especially when cells become lysed. 細胞の生存や増殖に異常が無いことを確認し、細胞洗浄の操作は穏やかに行ってください。We also recommend limiting the room temperature incubation time of the cell suspension with Concanavalin A beads to 5 minutes. Resting the tubes at the appropriate incubation temperatures in lieu of rotating or rocking the tubes during the incubations for cell-bead binding, antibody binding, and pAG-Tn5 enzyme binding helps to mitigate bead clumping and does not have any adverse effects on sequencing depth, identified peak numbers, or binding patterns.

しかしながら、CSTの社内試験では、ビーズの凝集は最終的な結果に悪影響をおよぼさないことが分かっています。

Last updated: 2024 年 4 月 17 日