Cat. # | Size | Qty. | Price | Inventory |
---|---|---|---|---|
74506C | 1 Kit (96 assays) |
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When ordering five or more kits, please contact us for processing time and pricing.
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REACTIVITY | H |
Product Includes | Volume | Solution Color | |||
---|---|---|---|---|---|
HS1 Rabbit mAb Coated Microwells | 96 tests | ||||
Phospho-HS1 (Tyr378/397) Rabbit Detection mAb | 1 ea | Red (Lyophilized) | |||
HRP Diluent | 5.5 ml | Red | |||
TMB Substrate 7004 | 11 ml | ||||
STOP Solution 7002 | 11 ml | ||||
Sealing Tape | 2 ea | ||||
ELISA Wash Buffer (20X) 9801 | 25 ml | ||||
Cell Lysis Buffer (10X) 9803 | 15 ml |
Product Information
The rapid protocol (RP) PathScan® RP Phospho-HS1 (Tyr378/397) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of HS1 protein phosphorylated at Tyr378/397 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-HS1 (Tyr378/397) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-HS1 (Tyr378/397). Learn more about your ELISA kit here.
*Antibodies in this kit are custom formulations specific to kit.
NOTE: This protocol is for PathScan® kits that use an HRP directly conjugated to the detection antibody (Rapid Protocol), rather than a 2-step method where the detection antibody and a secondary-HRP are added sequentially.
NOTE: Prepare solutions with deionized/purified water or equivalent.
For adherent cells
For suspension cells
NOTE: Equilibrate all materials and prepared reagents to room temperature prior to running the assay.
NOTE: Initial color of positive reaction is blue, which changes to yellow upon addition of STOP Solution.
created July 2020
Protocol Id: 2144
HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).
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