Flow cytometric analysis of 293T cells, mock transfected (blue) or transfected with a construct expressing an HA-tagged protein (green), using HA-Tag (C29F4) Rabbit mAb (PE-Cy7® Conjugate) (solid lines) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE-Cy7® Conjugate) #97492 (dashed lines).
This Cell Signaling Technology antibody is conjugated to phycoerythrin in combination with cyanine 7 (PE-Cy7®) and tested in-house for direct flow cytometric analysis in human cells.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
HA-Tag (C29F4) Rabbit mAb (PE-Cy7® Conjugate) detects exogenously expressed proteins containing the HA epitope tag.Species Reactivity:
All Species Expected
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.