|200 ug/ml||Endogenous||Rat IgG2b|
Flow cytometric analysis of live mouse splenocytes using CD45 (30-F11) Rat mAb (PE-Cy7® Conjugate) #60943 (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (PE-Cy7® Conjugate) (dashed line).Learn more about how we get our images.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.
Directions for Use: Important: Dilute this control antibody to the same concentration (not dilution) as specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rat IgG isotype control are used.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
The antibody is not directed against any known antigen. It functions as an isotype control for rat monoclonal antibodies.
This Cell Signaling Technology antibody is conjugated to PE-Cy7® under optimal conditions and tested in-house for direct flow cytometric analysis in mouse cells.
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Cy and CyDye are registered trademarks of GE Healthcare.
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