Western blot analysis of extracts from NIH/3T3 cells, prepared in lysis buffer in the absence of phosphatase inhibitors (left) or with Phosphatase Inhibitor Cocktail (100X) #5870 added (right), and incubated at 37ºC for the indicated time points, using Phospho-PKC (pan) (ΒII Ser660) Antibody #9371. In the absence of phosphatase inhibitors, Phospho-PKC signal fades within 3 hrs. after harvest, indicating protein degradation. In the presence of the phosphatase inhibitor cocktail, the Phospho-PKC degradation is slowed significantly and signal is still present at 24 hrs. following harvest.
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