|Product Includes||Quantity (with Count)||Solution Color|
|BrdU||1 x 150 µl|
|Fixing/denaturing Solution||2 x 25 ml|
|BrdU Mouse Detection mAb||1 x 500 µl||Green|
|Anti-mouse IgG, HRP-Linked Antibody||1 x 500 µl||Red|
|Detection Antibody Diluent||1 x 50 ml||Green|
|HRP Diluent||1 x 50 ml||Red|
|Luminol/Enhancer Solution||1 x 25 ml|
|Stable Peroxide Buffer||1 x 25 ml|
|20X Wash Buffer||1 x 50 ml|
posted October 2012
Protocol Id: 8
All Species Expected
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4).
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