注目情報はこちら >>
60721
53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free) #60721

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HCT 116 cells, wild-type (-) or 53BP1 knockdown (+), using 53BP1 (E7N5D) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using 53BP1 (E7N5D) XP® Rabbit mAb (left) or 53BP1 Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human 53BP1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 53BP1 (E7N5D) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HCT 116 cell pellet wild-type (left, positive) or 53BP1 knockdown (right, low-expressing) using 53BP1 (E7N5D) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the tongue using 53BP1 (E7N5D) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using 53BP1 (E7N5D) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using 53BP1 (E7N5D) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using 53BP1 (E7N5D) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HCT 116 cells, either wild-type (left) or 53BP1 knockdown (right), using 53BP1 (E7N5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 2: 53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HeLa cells either untreated (left) or treated with Actinomycin D #15021 (5 µg/mL, 2 hr) (right) using 53BP1 (E7N5D) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
To Purchase # 60721
製品# サイズ 数量 価格 在庫
60721SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 450
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #88439. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #88439

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

53BP1 (E7N5D) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total 53BP1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1190 of human 53BP1 protein.

Background

p53-binding protein 1 (53BP1) was originally identified as a p53 binding partner that could enhance the transcriptional activity of p53 (1,2). 53BP1 consists of two BRCA1 carboxy terminal (BRCT) domains that allow for binding to p53 and a separate domain responsible for binding to phosphorylated histone H2A.X (3). 53BP1 rapidly translocates to nuclear foci following treatment of cells with ionizing radiation (IR) or radiomimetic agents that cause DNA double strand breaks (DSBs) (4,5). Because of this localization to DSBs and homology to the yeast protein Rad9, a role for 53BP1 in DSB repair has been proposed. Recruitment of 53BP1 to sites of DNA damage has been demonstrated to be independent of ATM, NBS1, and DNA-PK (4) and retention of 53BP1 at DNA breaks requires phosphorylated H2A.X (6). In cells lacking 53BP1, phosphorylation of ATM substrates is reduced, suggesting that 53BP1 is upstream of ATM (7). In response to IR, phosphorylation of 53BP1 at serines 6, 25, 29, and 784 by ATM has been demonstrated, but phosphorylation at these sites is not required for localization of 53BP1 to sites of DSBs (6). Phosphorylation of 53BP1 at Ser1618 has been reported to be enriched in human cells arrested in mitosis (8).
  1. Iwabuchi, K. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 6098-102.
  2. Iwabuchi, K. et al. (1998) J. Biol. Chem. 273, 26061-8.
  3. Mochan, T.A. et al. (2004) DNA Repair (Amst) 3, 945-52.
  4. Schultz, L.B. et al. (2000) J. Cell Biol. 151, 1381-90.
  5. Anderson, L. et al. (2001) Mol. Cell. Biol. 21, 1719-29.
  6. Ward, I.M. et al. (2003) J. Biol. Chem. 278, 19579-82.
  7. DiTullio, R.A. et al. (2002) Nat. Cell Biol. 4, 998-1002.
  8. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Powered by Translations.com GlobalLink OneLink SoftwarePowered By OneLink