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50513
α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free) #50513

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Western blotting analysis of extracts from various human, mouse, and rat tissues using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (upper) and GAPDH #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human appendix using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse small intestine using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse small intestine (left) and skeletal muscle (right) using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
To Purchase # 50513SF
製品番号 サイズ 価格 在庫
50513SF
100 µg

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 42
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #19245. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #19245

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

α-Smooth Muscle Actin (D4K9N) XP® (BSA and Azide Free) Rabbit mAb recognizes endogenous levels of total α-smooth muscle protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).
  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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For Research Use Only. Not For Use In Diagnostic Procedures.
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