Render Target: SSR
Render Timestamp: 2024-12-10T20:03:12.467Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:53:28.756
Product last modified at: 2024-09-30T08:01:57.921Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AFAP1L2/XB130 (D1B5) Rabbit mAb #12684

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AFAP1L2/XB130 (D1B5) Rabbit mAb recognizes endogenous levels of total AFAP1L2/XB130 protein. In some cell extracts the antibody recognizes an 80 kDa background protein of unknown origin.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly638 of human AFAP1L2/XB130 protein.

    Background

    The actin-filament associated protein (AFAP) family consists of AFAP1, AFAP1L1, and AFAP1L2/XB130, a group of structurally similar proteins that play distinct roles in the regulation of cytoskeletal dynamics and signal transduction. Actin filament-associated protein 1-like 2 (AFAP1L2, XB130) is an adaptor protein that regulates signaling downstream of multiple kinases, including Src, Akt, and the thyroid specific kinase RET/PTC (1-3). Through these pathways, AFAP1L2/XB130 mediates transcriptional regulation, cell proliferation, motility, and microRNA expression (4,5). Research has shown that AFAP1L2/XB130 is involved in the proliferation and survival of thyroid tumor cells (6), and may have value in gastric cancer prognosis (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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