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97279
Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free) #97279

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  1. WB
  2. IHC
  3. F
Western Blotting Image 1: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Aiolos (D1C1E) Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from U-937 cells and RPMI-8226 cells, untreated (-) or lenalidomide-treated (2 μM, 24 hr; +), using Aiolos (D1C1E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8547 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Aiolos (D1C1E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Aiolos (D1C1E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of fixed (2% formaldehyde) and permeabilized (0.1% Triton X-100) mouse splenocytes using Aiolos (D1C1E) Rabbit mAb and co-stained with a CD19 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. Data were generated using the standard formulation of this product.
Flow Cytometry Image 2: Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of U-937 cells (blue), untreated RPMI-8226 cells (green), or lenalidomide-treated RPMI-8226 cells (2 μM, 24 hr; red), using Aiolos (D1C1E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 97279
製品# サイズ 数量 価格 在庫
97279SF
100 µg

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 50,58,65
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #15103. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #15103.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Aiolos (D1C1E) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Aiolos protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein.

Background

Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). Ikaros family proteins control lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, and elevated levels of IgG and IgE. In addition, Aiolos mice develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Aberrant expression of Aiolos in transformed epithelial cells promotes anchorage independence through downregulation of adhesion-related genes (6). Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (7-9).
  1. Morgan, B. et al. (1997) EMBO J 16, 2004-13.
  2. Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
  3. Kim, J. et al. (1999) Immunity 10, 345-55.
  4. Wang, J.H. et al. (1998) Immunity 9, 543-53.
  5. Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
  6. Li, X. et al. (2014) Cancer Cell 25, 575-89.
  7. Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
  8. Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
  9. Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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