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30797
Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free) #30797

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Annexin A1 (D5V2T) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human colon using Annexin A1 (D5V2T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma of the ovary using Annexin A1 (D5V2T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded A549 (left) and LNCaP (right) cell pellets using Annexin A1 (D5V2T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human spleen using Annexin A1 (D5V2T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of A549 (positive, left) and 293T (negative, right) cells using Annexin A1 (D5V2T) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of RL cells (blue) and Jurkat cells (green) using Annexin A1 (D5V2T) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 30797
製品# サイズ 数量 価格 在庫
30797SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 38
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #32934. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #32934

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Annexin A1 (D5V2T) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total annexin A1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human annexin A1 protein.

Background

The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).

使用に関する制限

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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected]
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