Render Target: SSR
Render Timestamp: 2024-12-10T20:03:57.952Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:47.351
Product last modified at: 2024-09-30T08:01:32.600Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AP1S1 (F8K5S) Rabbit mAb #32979

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AP1S1 (F8K5S) Rabbit mAb recognizes endogenous levels of total AP1S1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AP1S1 protein.

    Background

    The adaptor protein complex 1 (AP-1) is a heterotetrameric protein complex composed of two large adaptins (AP1G1 and AP1B1), a medium adaptin (AP1M1 or AP1M2), and one of several small adaptins (AP3S1, AP1S2, or AP1S3). AP-1 complexes containing AP1M1 are ubiquitously expressed, while complexes containing AP1M2 display a more restricted tissue expression pattern. Research studies have demonstrated that the AP-1 complex is localized to the trans-Golgi network and recycling endosomes where it regulates the bidirectional trafficking of cargo proteins between these subcellular compartments as well as the biogenesis of secretory vesicles (1-3).

    AP1S1 is one of three possible small adaptin subunits capable of being incorporated into the AP-1 heterotetrameric complex. Mutations in AP1S1 contribute to MEDNIK syndrome, which is characterized by a defect in copper metabolism due to aberrant sorting and trafficking of copper pumps ATP7A and ATP7B (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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