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92516
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit #92516

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ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 1
Confocal immunofluorescent analysis of fixed frozen hippocampus from C57BL/6 (left) and APOE4 knock-in (hu/hu, model #1549-F, right) mice labeled with ApoE (pan) (D7I9N) Rabbit mAb (top, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with GFAP (GA5) Mouse mAb (Alexa Fluor® 594 Conjugate) #8152 (bottom, red), and AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #89851 (bottom, blue pseudocolor). Mice from Taconic Biosciences, Inc.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 2
CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and either CREB (48H2) Rabbit mAb or Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across NR4A3 gene.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 3
Western blot analysis of extracts from mouse brain, rat brain, and rat prefrontal cortex tissues using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 4
Western blot analysis of cell extracts from Hep G2 cells treated with Brefeldin A #9972 (10 ng/ml, 90 min) and human cerebellum using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 5
Immunohistochemical analysis of paraffin-embedded human kidney using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 6
Western blot analysis of extracts from various cell lines and tissues using LRP1 (E2Q7S) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 7
Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 8
Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb (upper) or PSD95 (D74D3) XP® Rabbit mAb #3409 (lower).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 9
Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 10
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 11
Western Blot analysis of extracts from SK-N-MC, COS, NIH/3T3, C6 and Drosophila S2 cells, using CREB (48H2) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 12
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb (upper) or CREB (48H2) Rabbit mAb #9197 (lower).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 13
Western blot analysis of cell extracts from 293T cells, treated with Brefeldin A #9972 (10 ng/ml, 90 min; +) and mock transfected (-) or transfected with a construct expressing full-length human ApoE2 (hApoE2; +), ApoE3 (hApoE3; +), or ApoE4 (hApoE4; +), using ApoE (pan) (D7I9N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 14
Immunohistochemical analysis of paraffin-embedded liver (left) or brain (right) from C57BL/6NTac (wt/wt, model #B6-F, top) and APOE4 knock-in (hu/hu, model #1549-F, bottom) mice using ApoE (pan) (D7I9N) Rabbit mAb. Mice from Taconic Biosciences, Inc.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 15
Confocal immunofluorescent analysis of MEF-1 cells (left, LRP1 (+/+), positive) or PEA 13 cells (right, LRP1 (-/-), negative) using LRP1 (E2Q7S) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 16
Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human cerebellum using Synapsin-1 (D12G5) XP® Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 18
Western blot analysis of extracts from mouse brain, untreated (-) or λ-phosphatase-treated (+), using Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb (upper) and AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb #13185 (lower).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 19
Immunoprecipitation of CREB from SK-N-MC extracts. Lane 1 is CREB (48H2) Rabbit mAb, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is 10% input. Western blot analysis was perfomed using CREB (86B10) Mouse mAb #9104. Anti-mouse IgG, HRP-linked Antibody #7076 was used as a secondary antibody.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 21
Immunoprecipitation of AMPA Receptor 1 (GluA1) from mouse brain extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 22
Immunoprecipitation of ApoE from Hep G2 cells treated with Brefeldin A #9972 (10 ng/ml, 90 min), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ApoE (pan) (D7I9N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human spleen using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded mouse cortex using Synapsin-1 (D12G5) XP® Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded mouse lung using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded human small intestine using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded rat retina using Synapsin-1 (D12G5) XP® Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-CREB (Ser133) (87G3) Rabbit mAb in the presence of control peptide (left) or Phospho-CREB (Ser133) Blocking Peptide #1090 (right).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 31
Confocal immunofluorescent analysis of mouse hippocampus using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human skin using ApoE (pan) (D7I9N) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 33
Confocal immunofluorescent analysis of mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using CREB (48H2) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 35
Immunohistochemical analysis of paraffin-embedded SK-N-MC cells, untreated (left) or IBMX- and forskolin-treated (right), showing induced nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left-top) or transfected with human ApoE isoforms ApoE2 (right-top), ApoE3 (left-bottom), and ApoE4 (right-bottom) using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 39
Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or 293T cell pellet (right, negative) using ApoE (pan) (D7I9N) Rabbit mAb.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 40
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® #4084 (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 41
Confocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 42
Confocal immunofluorescent analysis of Hep G2 cells (left, positive) and 293T cells (right, negative) using ApoE (pan) (D7I9N) Rabbit mAb #13366 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 43
Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 44
Confocal microscopic images of SK-N-MC cells showing nuclear stain after 25 minute treatment with Forskolin and IBMX using Phospho-CREB (Ser133) (87G3) Rabbit mAb (left, red) compared to untreated cells (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 45
Flow cytometric analysis of Jurkat cells using CREB (48H2) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 46
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 (500nM) and Ionomycin #9995 (1uM,4hrs;green) using Phospho-CREB (Ser133) (87G3) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 47
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 μM) for 1h and either Phospho-CREB (Ser133) (87G3) Rabbit mAb or CREB (48H2) Rabbit mAb (#9197), using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across NR4A3, a known target gene of both Phospho-CREB and CREB (see additional figure containing ChIP-qPCR data).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 48
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells, treated with Forskolin #3828 (30 μM) for 1h and either 10 μl of CREB (48H2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 49
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 μM) for 1h and either Phospho-CREB (Ser133) (87G3) Rabbit mAb or CREB (48H2) Rabbit mAb (#9197), using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 9 (upper), including NR4A3 (lower), a known target gene of both Phospho-CREB and CREB (see additional figure containing ChIP-qPCR data).
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 50
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 μM) for 1h and either Phospho-CREB (Ser133) (87G3) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 51
Western blot analysis of extracts from HepG2 cells (lane 1), 293T mock transfected (lane 2) or transiently transfected with a construct expressing ApoE4 (lane 3), whole liver extracts from wild type C57BL/6NTac model #B6-F mice (lane 4), or ApoE4 knock-in (model #1549-F) (lane 5), whole brain extracts from wild type C57BL/6NTac mice (lane 6), or ApoE4 knock-in (lane 7) using ApoE (pan) (D7I9N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Mice from Taconic Biosciences, Inc.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 52
CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across NR4A3 gene.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 53
CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (ChIP-Seq, CUT&RUN) #56795. The figures show binding across chromosome 9 (upper), including NR4A3 (lower) gene.
ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit: Image 54
CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and either Phospho-CREB (Ser133) (87G3) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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To Purchase # 92516
製品# サイズ 数量 価格 在庫
92516T		 1 Kit  (9 x 20 microliters)

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
LRP1 (E2Q7S) Rabbit mAb 26387 20 µl
  • WB
  • IF
 H M R 85 Rabbit IgG
ApoE (pan) (D7I9N) Rabbit mAb 13366 20 µl
  • WB
  • IP
  • IHC
  • IF
 H 35 Rabbit IgG
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl
  • WB
  • IF
 H M R 95 Rabbit IgG
Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb 45737 20 µl
  • WB
 H M R 95 Rabbit IgG
Synapsin-1 (D12G5) XP® Rabbit mAb 5297 20 µl
  • WB
  • IP
  • IHC
  • IF
 H M R 77 Rabbit IgG
AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb 13185 20 µl
  • WB
  • IP
  • IF
 M R 100 Rabbit IgG
Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb 75574 20 µl
  • WB
  • IP
 H M 100 Rabbit IgG
CREB (48H2) Rabbit mAb 9197 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
  • C&R
 H M R Mk Dm 43 Rabbit IgG
Phospho-CREB (Ser133) (87G3) Rabbit mAb 9198 20 µl
  • WB
  • IHC
  • IF
  • F
  • ChIP
  • C&R
 H M R 43 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit provides an economical means of detecting key synaptic signaling pathways in response to ApoE-mediated LRP1 activation by western blot. The kit includes enough antibodies to perform at least two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each total antibody in the ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit detects endogenous levels of its target protein. LRP1 (E2Q7S) Rabbit mAb detects endogenous levels of the β subunit of LRP1. ApoE (pan) (D7I9N) Rabbit mAb also recognizes overexpressed ApoE2, ApoE3, and ApoE4 proteins. CREB (48H2) Rabbit mAb does not cross-react with other ATF/CREB family members. The antigen for Synapsin-1 (D12G5) XP® Rabbit mAb is 100% conserved between human synapsin-1a and synapsin-1b. Each phospho-specific antibody in the ApoE Synaptic Formation and Signaling Pathway Antibody Sampler Kit detects endogenous levels of CREB only when phosphorylated at Ser133, PSD95 protein only when phosphorylated at Ser295, and AMPA Receptor 1 (GluA1) protein only when phosphorylated at Ser831. Phospho-CREB (Ser133) (87G3) Rabbit mAb also detects the phosphorylated form of the CREB-related protein, ATF-1. While Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb refers to Ser831, consistent with the literature, it is Ser849 in the UniProt sequence P42261.

Source / Purification

Monoclonal antibodies to total proteins are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu4488 of human LRP1 protein, Pro285 of human ApoE protein,Gln53 of human PSD95 protein, Gln483 of human synapsin-1 protein, Ala275 of human AMPA Receptor 1 (GluA1) protein, and recombinant protein specific to the amino terminus of human CREB-1 protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser295 of human PSD95 protein, Ser831 of human AMPA Receptor 1 (GluA1) protein, and Ser133 of human CREB protein.

Background

Low density lipoprotein receptor related protein 1 (LRP1) is a type I transmembrane receptor that mediates the endocytosis of various ligands, including apolipoproteins and tau. Both proteins are genetically and pathologically linked to Alzheimer’s disease (AD) (1,2). Human apolipoprotein E (ApoE) is a component of circulating lipoproteins when three human genetic ApoE variants, ApoE2, ApoE3, and ApoE4, exhibit distinct receptor-binding properties and differentially contribute to AD progression through a cellular mechanism that is poorly understood (2). Altered synaptic signaling is one proposed mechanism that contributes to altered neuronal function, which correlates with disease (3-5). Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins that functions as a scaffolding protein to promote assembly and function of the postsynaptic density complex (6,7). At the presynapse, synapsins function to regulate neurotransmitter release (8,9). Dynamic phosphorylation of PSD95 at Ser295 reflects synaptic signaling that may alter synaptic function (10). In addition to PSD95, postsynaptic glutamate receptors, including AMPA-(α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors, can be directly phosphorylated. Phosphorylation of AMPA Receptor 1 (GluA1) at either Ser831 or Ser845 alters AMPA receptor ion channel function to change synaptic efficacy (11). CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+,stress signaling, as well as synaptic signaling (3). 

Pathways

Explore pathways related to this product.

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