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84441
β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free) #84441

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame deletion in the gene encoding β-Catenin (lane 2), using β-Catenin (D10A8) XP® Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). The change in molecular weight in the mutated HeLa cells is consistent with an in-frame deletion. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HeLa (left, positive) and NCI-H28 (right, negative) cells using β-Catenin (D10A8) XP® Rabbit mAb (green) and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of NCI-H28 cells (green) and HeLa cells (blue) using β-Catenin (D10A8) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 84441
製品# サイズ 数量 価格 在庫
84441SF
100 µg

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 92
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #8480. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.



This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #8480.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total β-catenin protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology:

Zebrafish, Bovine, Pig, Horse, Guinea Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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For Research Use Only. Not For Use In Diagnostic Procedures.
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