Render Target: SSR
Render Timestamp: 2024-12-10T20:05:35.000Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:15:46.500
Product last modified at: 2024-05-30T07:10:54.114Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

BCAR3 Antibody #24032

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 95
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    BCAR3 antibody recognizes endogenous levels of total BCAR3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro526 of human BCAR3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    BCAR3 is a member of the novel SH2-containing protein (NSP) family (1). It was identified as a gene product involved in anti-estrogen resistance in the context of breast cancer (2). Like other members of this family, BCAR3 has been shown to interact with the family member, CAS. The C terminal Cdc25 homolgy domain of BCAR3 interacts tightly with the FAT domain of p130Cas (3) and promotes the association of p130cas with Src kinase (4) to activate related signaling pathways. Overexpression of BCAR3 leads to the activation of a wide range of downstream signaling proteins including PI3K, rac, PAK1, and cyclin D1 (5-7). The main role of BCAR3 is to promote cell motility and regulate cytoskeletal remodeling and adhesion through its effect on p130cas and Src kinase (8-10). BCAR3 also has been implicated in playing an inhibitory role on TGF-β/SMAD signaling, which is associated with favorable disease outcomes (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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