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56263
BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free) #56263

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  1. IHC
Immunohistochemistry Image 1: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using BCL6 (E5I8I) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using BCL6 (E5I8I) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma using BCL6 (E5I8I) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using BCL6 (E5I8I) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human uterine papillary serous carcinoma using BCL6 (E5I8I) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using BCL6 (E5I8I) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using BCL6 (E5I8I) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Ramos cell pellet (left, positive) or RD cell pellet (right, negative) using BCL6 (E5I8I) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 56263
製品# サイズ 数量 価格 在庫
56263SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #89369. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #89369.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

BCL6 (E5I8I) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total BCL6 protein. It is predicted to detect both known isoforms of human BCL6. Non-specific, non-nuclear staining was observed in human and mouse smooth muscle. Additional non-specific staining was observed in other mouse tissues.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro319 of human BCL6 protein.

Background

Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkin's lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).
  1. Ye, B.H. et al. (1993) Science 262, 747-50.
  2. Onizuka, T. et al. (1995) Blood 86, 28-37.
  3. Zollman, S. et al. (1994) Proc Natl Acad Sci U S A 91, 10717-21.
  4. Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75.
  5. Shvarts, A. et al. (2002) Genes Dev 16, 681-6.
  6. Moriyama, M. et al. (1997) Oncogene 14, 2465-74.
  7. Niu, H. et al. (1998) Genes Dev 12, 1953-61.
  8. Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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