Render Target: SSR
Render Timestamp: 2024-12-10T20:05:41.471Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:58:09.513
Product last modified at: 2024-10-31T12:15:12.450Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BCOR (E6V3R) Rabbit mAb #63972

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 210, 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    BCOR (E6V3R) Rabbit mAb recognizes endogenous levels of total BCOR protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCOR protein.

    Background

    BCL6 corepressor protein (BCOR) is a ubiquitously expressed transcriptional repressor that is recruited to target genes by BCL6 and other transcription factors (1). BCOR interacts with and recruits histone deacetylase (HDAC) and DNA methyltransferase (DNMT) proteins to facilitate localized histone deacetylation and DNA methylation, resulting in repression of gene expression (1,2). Mutations and translocations involving BCOR have been observed in acute myeloid leukemia, bone sarcoma, and medulloblastoma, and mutations in BCOR are known to cause X-linked Oculofaciocardiodental and Lenz microphthalmia syndromes (3-6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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