Bim (C34C5) Rabbit mAb (BSA and Azide Free) #26184

Product Usage Information

This product is the carrier-free version of product #2933. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #2933.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Bim (C34C5) Rabbit mAb (BSA and Azide Free) detects endogenous levels of total Bim (EL, L and S isoforms) protein.

Species Reactivity:

Human, Mouse, Rat

Species predicted to react based on 100% sequence homology:

Monkey, Bovine, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of Bim.

Background

Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: Bim_EL, Bim_L, and Bim_S (1). The shortest form, Bim_S, is the most cytotoxic and is generally only transiently expressed during apoptosis. The Bim_EL and Bim_L isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.

1. O'Connor, L. et al. (1998) EMBO J 17, 384-95.
2. Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40.
3. Bouillet, P. et al. (2002) Nature 415, 922-6.
4. Whitfield, J. et al. (2001) Neuron 29, 629-43.
5. Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4.
6. Ley, R. et al. (2003) J Biol Chem 278, 18811-6.
7. Puthalakath, H. et al. (1999) Mol Cell 3, 287-96.
8. Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7.
9. Putcha, G.V. et al. (2003) Neuron 38, 899-914.

Pathways & Proteins

Explore pathways + proteins related to this product.

Select Your Pathway/Protein Apoptosis Regulation Growth And Differentiation Control by MAPKs Inhibition of Apoptosis Mitochondrial Control of Apoptosis PI3K/Akt Signaling Protein: O43521

Database Links

UniProt ID: O43521

Entrez-Gene Id: 10018

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