For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn96 of human CARD14 protein.
CARD14 (caspase recruitment domain-containing protein 14) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, functioning as molecular scaffolds for the assembly of multiprotein complexes (1-3). CARD14 has been shown to regulate BCL10 function, leading to NF-κB activation (1-3). It also induces activation of p38 and JNK MAP kinase pathways via interaction with MALT1 (4). Full-length CARD14, CARD14fl or isoform 1, encodes both an N-terminal CARD domain necessary for NF-kB activation, and a C-terminal tripartite MAGUK domain (PDZ/SH3/GUK). A shorter isoform, CARD14sh, encodes the CARD domain but not the tripartite domain (1,2). Mutations of CARD14 are associated with psoriasis and psoriatic arthritis (2,3).
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