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For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCDC22 protein.
Coiled-coil domain-containing protein 22 (CCDC22) is a highly conserved protein that harbors an N-terminal calponin homology-like (NN-CH) domain and a C-terminal coiled-coil domain. CCDC22 is a core subunit of the COMMD/CCDC93/CCDC22 (CCC) protein complex, which also consists of CCDC93 and any one of ten COMMD proteins. The CCC complex, which is a subcomplex of the commander complex, plays a key role in protecting the stability and promoting the endosomal recycling of cell surface transmembrane proteins, such as integrins, LDLR, and the ATP7A copper transporter (1-3). Research studies have also demonstrated that as part of a ubiquitin ligase complex, CCDC22 activates proinflammatory NF-κB signaling by promoting the ubiquitin-dependent degradation of IκB proteins (4).
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