We recommend using 0.25-1.0 μg of antibody per test. The optimal dilution is assay dependent and may require titration.
|Flow Cytometry||1:50 - 1:200|
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze.
CD16/CD32 (2.4G2) Rat mAb recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32.
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.
CD64 (FcgammaRI), CD32 (FcgammaRII) and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32 and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).
The 2.4G2 clone is widely used as a pre-treatment reagent to reduce non-specific binding of mouse Fc-receptors (4).
Explore pathways + proteins related to this product.