We recommend using 0.25-1.0 μg of antibody per test. The optimal dilution is assay dependent and may require titration.
|Flow Cytometry||1:50 - 1:200|
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze.
CD16/CD32 (2.4G2) Rat mAb recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32.Species Reactivity:
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.
CD64 (FcgammaRI), CD32 (FcgammaRII) and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32 and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).
The 2.4G2 clone is widely used as a pre-treatment reagent to reduce non-specific binding of mouse Fc-receptors (4).
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