Render Target: SSR
Render Timestamp: 2025-01-30T17:02:10.193Z
Commit: 1bba917eefc12d62e72a522121e2774ffbd0ee36
XML generation date: 2024-09-30 01:58:34.540
Product last modified at: 2025-01-01T09:01:32.744Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD300a (E6B9T) Rabbit mAb #87572

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD300a (E6B9T) Rabbit mAb recognizes endogenous levels of total CD300a protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human CD300a protein.

    Background

    CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail (1,2,5). Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granulocytes, mast cells, and subsets of B cells (1-5). Human, but not mouse, CD300a is also expressed by unstimulated NK cells and subsets of T cells (1,5). Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation (1,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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