Render Target: SSR
Render Timestamp: 2025-01-30T17:09:55.927Z
Commit: 1bba917eefc12d62e72a522121e2774ffbd0ee36
XML generation date: 2024-09-30 01:58:53.083
Product last modified at: 2025-01-01T09:05:39.363Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HAT1 (E7Y8Q) Rabbit mAb #41490

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 49
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HAT1 (E7Y8Q) Rabbit mAb recognizes endogenous levels of total HAT1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val412 of human HAT1 protein.

    Background

    Histone acetyltransferase 1 (HAT1) is a member of the GCN5 family of acetyltransferases that can acetylate Lys5 and Lys12 on newly generated histone H4, a process that is essential for mammalian development (1-5). HAT1 binds to the H4 gene promoter, where it facilitates the production and acetylation of new histones. This process is strongly dependent on glucose availability, suggesting HAT1 plays a role in nutrient sensing (6). HAT1 has also been shown to contribute to recovery from replication-based DNA damage by incorporating H4Lys5/Lys12 acetylated histones at the sites of double-stranded breaks (7-9). HAT1 can acetylate non-histone proteins as well, and has been shown to play a key role in NF-kB signaling by acetylating PLZF, which in turn forms a complex with p50 to limit the production of cytokines (10). HAT1 overexpression has also been implicated in many cancer types (11-13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
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