Name: Human Exhausted T Cell Antibody Sampler Kit
Brand: Cell Signaling Technology
Price: 129000.00 USD
Availability: InStock

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Human Exhausted T Cell Antibody Sampler Kit: Image 1 — Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using LAG3 (D2G4O™) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Human Exhausted T Cell Antibody Sampler Kit: Image 2 — Western blot analysis of extracts from HDLM-2, HuT 78, and Jurkat cells using LAG3 (D2G4O™) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 3 — Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide^® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 4 — Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 5 — Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 6 — Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Human Exhausted T Cell Antibody Sampler Kit: Image 7 — Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using LAG3 (D2G4O™) XP® rabbit mAb (orange), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).

Human Exhausted T Cell Antibody Sampler Kit: Image 8 — Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Human Exhausted T Cell Antibody Sampler Kit: Image 9 — Western blot analysis of total cell lysates from HT29, Colo201, Jurkat and mouse thymocytes using TCF1/TCF7 (C63D9) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 10 — Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using TCF1/TCF7 (C63D9) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 11 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TCF1/TCF7 (C63D9) Rabbit mAb in the presence of control peptide (left) or TCF1/TCF7 blocking peptide #1007 (right).

Human Exhausted T Cell Antibody Sampler Kit: Image 12 — Immunohistochemical analysis of paraffin-embedded human tonsil using TCF1/TCF7 (C63D9) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 13 — Confocal immunofluorescent analysis of DLD-1 cells using TCF1/TCF7 (C63D9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red).

Human Exhausted T Cell Antibody Sampler Kit: Image 14 — Flow cytometric analysis of A20 cells (blue) and EL-4 cells (green) using TCF1/TCF7 (C63D9) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 15 — Chromatin immunoprecipitations were performed with cross-linked chromatin from mouse thymocytes and either TCF1/TCF7 (C63D9) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse LEF1 Upstream Primers #80993 and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Human Exhausted T Cell Antibody Sampler Kit: Image 16 — Flow cytometric analysis of human peripheral blood mononuclear cells gated on lymphocytes co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881 using Tox/Tox2 (E6G5O) Rabbit mAb (right) compared to concentration matched
Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 17 — Western blot analysis of extracts from various human cell lines using Tox/Tox2 (E6G5O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 18 — Flow cytometric analysis of RPMI 8226 cells (blue, negative) and SU-DHL-4 cells (green, positive) using Tox/Tox2 (E6G5O) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^®488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 19 — Western blot analysis of extracts from human, mouse, and rat tissues and mouse cell lines using Tox/Tox2 (E6G5O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 20 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human Tox protein (hTox-Myc/DDK), Myc/DDK-tagged full-length human Tox2 protein (hTox2-Myc/DDK), Myc/DDK-tagged full-length human Tox3 protein (hTox3-Myc/DDK), or Myc/DDK-tagged full-length human Tox4 protein (hTox4-Myc/DDK), using Tox/Tox2 (E6G5O) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 21 — Immunoprecipitation of Tox protein from SU-DHL-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900, and lane 3 is Tox/Tox2 (E6G5O) Rabbit mAb. Western blot analysis was performed using Tox/Tox2 (E6G5O) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 22 — Confocal immunofluorescent analysis of mouse thymus (left) and pancreas (right) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

Human Exhausted T Cell Antibody Sampler Kit: Image 23 — Confocal immunofluorescent analysis of rat thymus (left) and pancreas (right) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

Human Exhausted T Cell Antibody Sampler Kit: Image 24 — Confocal immunofluorescent analysis of mouse colon using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

Human Exhausted T Cell Antibody Sampler Kit: Image 25 — Confocal immunofluorescent analysis of SU-DHL-4 cells (left, positive) or A549 cells (right, negative) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

Human Exhausted T Cell Antibody Sampler Kit: Image 26 — Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated SU-DHL-4 cells and either Tox/Tox2 (E6G5O) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human HERC2 intron 1 primers, human WAPAL exon 1 primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Human Exhausted T Cell Antibody Sampler Kit: Image 27 — Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using TIM-3 (D5D5R™) XP^® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human Exhausted T Cell Antibody Sampler Kit: Image 28 — Immunohistochemical analysis of paraffin-embedded renal clear cell carcinoma using TIM-3 (D5D5R™) XP^® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Exhausted T Cell Antibody Sampler Kit: Image 29 — Western blot analysis of extracts from primary human CD4+ T cells and various cell lines using TIM-3 (D5D5R™) XP^® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human Exhausted T Cell Antibody Sampler Kit: Image 30 — Immunohistochemical analysis of paraffin-embedded human tonsil using TIM-3 (D5D5R™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 31 — Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK-tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 (D5D5R™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 32 — Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-3 (D5D5R™) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 33 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 (D5D5R™) XP^® Rabbit mAb. Note staining of alveolar macrophages.

Human Exhausted T Cell Antibody Sampler Kit: Image 34 — Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using TIM-3 (D5D5R™) XP^® Rabbit mAb. CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human Exhausted T Cell Antibody Sampler Kit: Image 35 — Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng TIM-3 (D5D5R™) XP® rabbit mAb (yellow), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Human Exhausted T Cell Antibody Sampler Kit: Image 36 — Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TIM-3 (D5D5R™) XP® rabbit mAb, PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan),and LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange).

Human Exhausted T Cell Antibody Sampler Kit: Image 37 — Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using TIM-3 (D5D5R™) XP® rabbit mAb (yellow), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Human Exhausted T Cell Antibody Sampler Kit: Image 38 — Western blot analysis of extracts from various cell lines using 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 39 — Immunoprecipitation of 2B4/SLAMF4/CD244 protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900, and lane 3 is 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb. Western blot analysis was performed using 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 40 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Human Exhausted T Cell Antibody Sampler Kit: Image 41 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either EOMES (D8D1R) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PRF1 Promoter Primers #9014, human CCL4 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Human Exhausted T Cell Antibody Sampler Kit: Image 42 — Western blot analysis of extracts from various cell lines using EOMES (D8D1R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 43 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either EOMES (D8D1R) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Sonication Chromatin IP Kit #56383. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PRF1 Promoter Primers #9014, human CCL4 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Human Exhausted T Cell Antibody Sampler Kit: Image 44 — Confocal immunofluorescent analysis of NK-92 cells (left, positive) and HeLa cells (right, negative) using EOMES (D8D1R) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Human Exhausted T Cell Antibody Sampler Kit: Image 45 — Flow cytometric analysis of human peripheral blood mononuclear cells, co-stained with a CD14 antibody (left) or a CD3 antibody (right), using EOMES (D8D1R) Rabbit mAb (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype control #3900 (bottom row). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 46 — Flow cytometric analysis of RPMI 8226 cells (blue) and NK-92 cells (green), using EOMES (D8D1R) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 47 — Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using PD-1 (D4W2J) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Exhausted T Cell Antibody Sampler Kit: Image 48 — Western blot analysis of extracts from human CD4⁺ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP^® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4⁺ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human Exhausted T Cell Antibody Sampler Kit: Image 49 — Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 50 — Immunoprecipitation of PD-1 protein from Molt-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900, and lane 3 is PD-1 (D4W2J) XP^® Rabbit mAb. Western blot analysis was performed using PD-1
(D4W2J) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 51 — Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using PD-1 (D4W2J) XP^®Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 52 — Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 53 — Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng PD-1 (D4W2J) XP® rabbit mAb (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Human Exhausted T Cell Antibody Sampler Kit: Image 54 — Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-1 (D4W2J) XP® rabbit mAb (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Human Exhausted T Cell Antibody Sampler Kit: Image 55 — Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells, untreated (left column) or treated with anti-CD3 (10ug/ml, 72hr) and anti-CD28 (5ug/ml, 72 hr; right column), using PD-1 (D4W2J) XP^® Rabbit mAb #86163 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (bottom row), and co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881. Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 56 — Western blot analysis of extracts from various human cells using CTLA-4 (E1V6T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4⁺ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human Exhausted T Cell Antibody Sampler Kit: Image 57 — Immunoprecipitation of CTLA-4 protein from CD4⁺ T cell extracts. CD4⁺ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900, and lane 3 is CTLA-4 (E1V6T) Rabbit mAb. Western blot analysis was performed using CTLA-4 (E1V6T) Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 58 — Western blot analysis of extracts from human CD8⁺ T cells and various human cell lines using TIGIT (E5Y1W) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Exhausted T Cell Antibody Sampler Kit: Image 59 — Immunoprecipitation of TIGIT protein from MJ [G11] cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900, and lane 3 is TIGIT (E5Y1W) XP^® Rabbit mAb. Western blot analysis was performed using TIGIT (E5Y1W) XP^® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 60 — Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left-top) or TIGIT-transfected (right-top), MJ [G11] cell pellet (left-bottom, positive), and purified CD8⁺human peripheral blood mononuclear cell pellet (right-bottom, positive), using TIGIT (E5Y1W) XP^® Rabbit mAb. CD8⁺ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (7 ng/mL).

Human Exhausted T Cell Antibody Sampler Kit: Image 61 — Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 62 — Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 63 — Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 64 — Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 65 — Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 66 — Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using TIGIT (E5Y1W) XP^® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (right).

Human Exhausted T Cell Antibody Sampler Kit: Image 67 — Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 68 — Immunohistochemical analysis of paraffin-embedded human tonsil using TIGIT (E5Y1W) XP^® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 69 — Confocal immunofluorescent analysis of human CD8⁺ T cells using TIGIT (E5Y1W) XP^® Rabbit mAb (green) and CD8α (RPA-T8) Mouse mAb (FITC Conjugate) #55397 (red pseudocolor). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue). CD8⁺ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (20 ng/mL).

Human Exhausted T Cell Antibody Sampler Kit: Image 70 — Confocal immunofluorescent analysis of MJ [G11] cells (left, positive) and IGROV-1 cells (right, negative) using TIGIT (E5Y1W) XP^® Rabbit mAb (green). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

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To Purchase # 81490T

製品番号	サイズ	価格	在庫
81490T	1 Kit (9 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Tox/Tox2 (E6G5O) Rabbit mAb 36778	20 µl	WB IP IF F ChIP	H M R	60-80	Rabbit IgG
TCF1/TCF7 (C63D9) Rabbit mAb 2203	20 µl	WB IP IHC IF F ChIP	H M	48, 50	Rabbit IgG
EOMES (D8D1R) Rabbit mAb 81493	20 µl	WB IF F ChIP	H	75, 85	Rabbit IgG
PD-1 (D4W2J) XP^® Rabbit mAb 86163	20 µl	WB IP IHC F	H	52-65	Rabbit IgG
CTLA-4 (E1V6T) Rabbit mAb 96399	20 µl	WB IP	H	25-30	Rabbit IgG
TIGIT (E5Y1W) XP^® Rabbit mAb 99567	20 µl	WB IP IHC IF	H	18, 30-40	Rabbit IgG
LAG3 (D2G4O™) XP^® Rabbit mAb 15372	20 µl	WB IHC	H	60-80	Rabbit IgG
TIM-3 (D5D5R™) XP^® Rabbit mAb 45208	20 µl	WB IP IHC F	H	45-70	Rabbit IgG
2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb 54560	20 µl	WB IP	H M	70-120	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB			Goat

Kit Usage Information

Protocols

2203: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic
7074: Western Blotting
15372: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin)
36778: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic
45208: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Flow Cytometry Live Cell Unconjugated Rabbit
54560: Western Blotting, Immunoprecipitation (Agarose)
81493: Western Blotting, Immunofluorescence, Flow, Sonication Chromatin Immunoprecipitation Protocol
86163: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Flow
96399: Western Blotting, Immunoprecipitation (Magnetic)
99567: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence

Product Description

The Human Exhausted T Cell Antibody Sampler Kit provides an economical means of characterizing the extent of exhaustion in T cells. This kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Human Exhausted T Cell Antibody Sampler Kit detects endogenous levels of its target human protein. Tox/Tox2 (E6G5O) Rabbit mAb does not cross-react with Tox3 or Tox4 proteins, but does cross-react with an unidentified protein of 50 kDa in some murine cell extracts. TCF1/TCF7 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. TIGIT (E5Y1W) XP^® Rabbit mAb cross-reacts with an unidentified protein of 42 kDa in some cell extracts. 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb cross-reacts with an unidentified protein of 18 kDa in some cell extracts.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln435 of human Tox protein, Pro96 of human TCF1/TCF7 protein, Pro180 of human EOMES protein, Ala274 of human PD-1 protein, Leu95 of human CTLA-4 protein, and Asp367 of human 2B4/SLAMF4/CD244 protein. Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human TIGIT protein, the extracellular domain of human TIM-3 protein, and the amino terminus of human LAG3 protein.

Background

Tox, TCF1/TCF7, and EOMES play key roles in T cell development. Tox is also induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion. TCF1/TCF7 preserves the effector function of exhausted T cells during viral infection or cancer. EOMES is a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells. Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection, and high levels of EOMES promotes T cell exhaustion. The dynamic expression of these transcription factors help characterize the extent to which a T cell is exhausted and will respond to antigen stimulation (1-5) PD-1 (PDCD1, CD279), CTLA-4 (CD152), TIGIT (VSIG9, VSTM3), TIM-3 (HAVCR2), LAG3 (CD223), and 2B4 (SLAMF4, CD244) are immune cell co-inhibitory receptors (also known as immune checkpoints) that negatively regulate T cell function and dampen the immune response to pathogens and cancer. In addition to activated T cells, PD-1 is expressed by activated B cells and monocytes. Following interaction with its ligands, PD-L1 and PD-L2, PD-1 is phosphorylated at ITIM and ITSM motifs leading to recruitment of protein tyrosine phosphatases SHP-1 and SHP-2 and suppression of TCR signaling. CTLA-4 protein is primarily expressed on T cells, including CD8+ cytotoxic T cells, CD4+ helper T cells, and CD4+/FoxP3+ regulatory T cells. CTLA-4 protein competes with CD28 for B7.1 (CD80) and B7.2 (CD86) binding at the cell surface, resulting in downregulation of T cell activity. TIGIT is expressed at low levels on subsets of T cells and natural killer (NK) cells, and is upregulated at the protein level following activation of these cells. TIGIT marks exhausted T cells in the tumor microenvironment and during human immunodeficiency virus (HIV) infection. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer. Tumor-infiltrating macrophages and dendritic cells also express TIM-3. LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, FoxP3+ T regulatory cells (Tregs), and NK cells. 2B4 is a heterophilic cell surface receptor expressed on a variety of immune cells, including NK cells, T cells, eosinophils, mast cells, and dendritic cells. 2B4 has been shown to have both immune stimulatory and inhibitory effects on cells. Co-expression of multiple immune checkpoints help characterize the extent to which a T cell is exhausted and will respond to antigen stimulation. Therapeutic blockade of several of these immune checkpoint receptors is a promising strategy for neoplastic intervention by enabling anti-tumor immune responses (6-13).

Pathways & Proteins

Explore pathways + proteins related to this product.

Database Links

UniProt ID: P36402 , Q96NM4 , O94900 , P16410 , O95936 , Q9BZW8 , Q495A1 , P18627 , Q15116 , Q8TDQ0

Entrez-Gene Id: 6932 , 84969 , 9760 , 1493 , 8320 , 51744 , 201633 , 3902 , 5133 , 84868

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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Human Exhausted T Cell Antibody Sampler Kit #81490