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81490
Human Exhausted T Cell Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Human Exhausted T Cell Antibody Sampler Kit #81490

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Human Exhausted T Cell Antibody Sampler Kit: Image 1
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using LAG3 (D2G4O™) XP® Rabbit mAb performed on the Leica® Bond™ Rx.
Human Exhausted T Cell Antibody Sampler Kit: Image 2
Western blot analysis of extracts from HDLM-2, HuT 78, and Jurkat cells using LAG3 (D2G4O™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 3
Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 4
Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 5
Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 6
Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).
Human Exhausted T Cell Antibody Sampler Kit: Image 7
Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using LAG3 (D2G4O™) XP® rabbit mAb (orange), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).
Human Exhausted T Cell Antibody Sampler Kit: Image 8
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).
Human Exhausted T Cell Antibody Sampler Kit: Image 9
Western blot analysis of total cell lysates from HT29, Colo201, Jurkat and mouse thymocytes using TCF1/TCF7 (C63D9) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using TCF1/TCF7 (C63D9) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 11
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TCF1/TCF7 (C63D9) Rabbit mAb in the presence of control peptide (left) or TCF1/TCF7 blocking peptide #1007 (right).
Human Exhausted T Cell Antibody Sampler Kit: Image 12
Immunohistochemical analysis of paraffin-embedded human tonsil using TCF1/TCF7 (C63D9) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 13
Confocal immunofluorescent analysis of DLD-1 cells using TCF1/TCF7 (C63D9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Human Exhausted T Cell Antibody Sampler Kit: Image 14
Flow cytometric analysis of A20 cells (blue) and EL-4 cells (green) using TCF1/TCF7 (C63D9) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 15
Chromatin immunoprecipitations were performed with cross-linked chromatin from mouse thymocytes and either TCF1/TCF7 (C63D9) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse LEF1 Upstream Primers #80993 and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Human Exhausted T Cell Antibody Sampler Kit: Image 16
Flow cytometric analysis of human peripheral blood mononuclear cells gated on lymphocytes co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881 using Tox/Tox2 (E6G5O) Rabbit mAb (right) compared to concentration matched

Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Exhausted T Cell Antibody Sampler Kit: Image 17
Western blot analysis of extracts from various human cell lines using Tox/Tox2 (E6G5O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 18
Flow cytometric analysis of RPMI 8226 cells (blue, negative) and SU-DHL-4 cells (green, positive) using Tox/Tox2 (E6G5O) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 19
Western blot analysis of extracts from human, mouse, and rat tissues and mouse cell lines using Tox/Tox2 (E6G5O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 20
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human Tox protein (hTox-Myc/DDK), Myc/DDK-tagged full-length human Tox2 protein (hTox2-Myc/DDK), Myc/DDK-tagged full-length human Tox3 protein (hTox3-Myc/DDK), or Myc/DDK-tagged full-length human Tox4 protein (hTox4-Myc/DDK), using Tox/Tox2 (E6G5O) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 21
Immunoprecipitation of Tox protein from SU-DHL-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Tox/Tox2 (E6G5O) Rabbit mAb. Western blot analysis was performed using Tox/Tox2 (E6G5O) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 22
Confocal immunofluorescent analysis of mouse thymus (left) and pancreas (right) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Human Exhausted T Cell Antibody Sampler Kit: Image 23
Confocal immunofluorescent analysis of rat thymus (left) and pancreas (right) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Human Exhausted T Cell Antibody Sampler Kit: Image 24
Confocal immunofluorescent analysis of mouse colon using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Human Exhausted T Cell Antibody Sampler Kit: Image 25
Confocal immunofluorescent analysis of SU-DHL-4 cells (left, positive) or A549 cells (right, negative) using Tox/Tox2 (E6G5O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Human Exhausted T Cell Antibody Sampler Kit: Image 26
Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated SU-DHL-4 cells and either Tox/Tox2 (E6G5O) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human HERC2 intron 1 primers, human WAPAL exon 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Human Exhausted T Cell Antibody Sampler Kit: Image 27
Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using TIM-3 (D5D5R™) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).
Human Exhausted T Cell Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded renal clear cell carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Human Exhausted T Cell Antibody Sampler Kit: Image 29
Western blot analysis of extracts from primary human CD4+ T cells and various cell lines using TIM-3 (D5D5R™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).
Human Exhausted T Cell Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded human tonsil using TIM-3 (D5D5R™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 31
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK-tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 (D5D5R™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb. Note staining of alveolar macrophages.
Human Exhausted T Cell Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using TIM-3 (D5D5R™) XP® Rabbit mAb. CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).
Human Exhausted T Cell Antibody Sampler Kit: Image 35
Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng TIM-3 (D5D5R™) XP® rabbit mAb (yellow), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).
Human Exhausted T Cell Antibody Sampler Kit: Image 36
Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TIM-3 (D5D5R™) XP® rabbit mAb, PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan),and LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange).
Human Exhausted T Cell Antibody Sampler Kit: Image 37
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using TIM-3 (D5D5R™) XP® rabbit mAb (yellow), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).
Human Exhausted T Cell Antibody Sampler Kit: Image 38
Western blot analysis of extracts from various cell lines using 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 39
Immunoprecipitation of 2B4/SLAMF4/CD244 protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb. Western blot analysis was performed using 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 40
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Human Exhausted T Cell Antibody Sampler Kit: Image 41
Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either EOMES (D8D1R) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PRF1 Promoter Primers #9014, human CCL4 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Human Exhausted T Cell Antibody Sampler Kit: Image 42
Western blot analysis of extracts from various cell lines using EOMES (D8D1R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 43
Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either EOMES (D8D1R) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Sonication Chromatin IP Kit #56383. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PRF1 Promoter Primers #9014, human CCL4 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Human Exhausted T Cell Antibody Sampler Kit: Image 44
Confocal immunofluorescent analysis of NK-92 cells (left, positive) and HeLa cells (right, negative) using EOMES (D8D1R) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).
Human Exhausted T Cell Antibody Sampler Kit: Image 45
Flow cytometric analysis of human peripheral blood mononuclear cells, co-stained with a CD14 antibody (left) or a CD3 antibody (right), using EOMES (D8D1R) Rabbit mAb (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (bottom row). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 46
Flow cytometric analysis of RPMI 8226 cells (blue) and NK-92 cells (green), using EOMES (D8D1R) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using PD-1 (D4W2J) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Human Exhausted T Cell Antibody Sampler Kit: Image 48
Western blot analysis of extracts from human CD4+ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).
Human Exhausted T Cell Antibody Sampler Kit: Image 49
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 50
Immunoprecipitation of PD-1 protein from Molt-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PD-1 (D4W2J) XP® Rabbit mAb. Western blot analysis was performed using PD-1

(D4W2J) XP® Rabbit mAb.

Human Exhausted T Cell Antibody Sampler Kit: Image 51
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using PD-1 (D4W2J) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 52
Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 53
Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng PD-1 (D4W2J) XP® rabbit mAb (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).
Human Exhausted T Cell Antibody Sampler Kit: Image 54
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-1 (D4W2J) XP® rabbit mAb (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).
Human Exhausted T Cell Antibody Sampler Kit: Image 55
Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells, untreated (left column) or treated with anti-CD3 (10ug/ml, 72hr) and anti-CD28 (5ug/ml, 72 hr; right column), using PD-1 (D4W2J) XP® Rabbit mAb #86163 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (bottom row), and co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881.  Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 56
Western blot analysis of extracts from various human cells using CTLA-4 (E1V6T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).
Human Exhausted T Cell Antibody Sampler Kit: Image 57
Immunoprecipitation of CTLA-4 protein from CD4+ T cell extracts. CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CTLA-4 (E1V6T) Rabbit mAb. Western blot analysis was performed using CTLA-4 (E1V6T) Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 58
Western blot analysis of extracts from human CD8+ T cells and various human cell lines using TIGIT (E5Y1W) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Exhausted T Cell Antibody Sampler Kit: Image 59
Immunoprecipitation of TIGIT protein from MJ [G11] cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TIGIT (E5Y1W) XP® Rabbit mAb. Western blot analysis was performed using TIGIT (E5Y1W) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Human Exhausted T Cell Antibody Sampler Kit: Image 60
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left-top) or TIGIT-transfected (right-top), MJ [G11] cell pellet (left-bottom, positive), and purified CD8+ human peripheral blood mononuclear cell pellet (right-bottom, positive), using TIGIT (E5Y1W) XP® Rabbit mAb. CD8+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (7 ng/mL).
Human Exhausted T Cell Antibody Sampler Kit: Image 61
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 62
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 63
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 64
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 65
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 66
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using TIGIT (E5Y1W) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Human Exhausted T Cell Antibody Sampler Kit: Image 67
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 68
Immunohistochemical analysis of paraffin-embedded human tonsil using TIGIT (E5Y1W) XP® Rabbit mAb.
Human Exhausted T Cell Antibody Sampler Kit: Image 69
Confocal immunofluorescent analysis of human CD8+ T cells using TIGIT (E5Y1W) XP® Rabbit mAb (green) and CD8α (RPA-T8) Mouse mAb (FITC Conjugate) #55397 (red pseudocolor). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). CD8+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (20 ng/mL).
Human Exhausted T Cell Antibody Sampler Kit: Image 70
Confocal immunofluorescent analysis of MJ [G11] cells (left, positive) and IGROV-1 cells (right, negative) using TIGIT (E5Y1W) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
To Purchase # 81490T
製品番号 サイズ 価格 在庫
81490T
1 Kit  (9 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Tox/Tox2 (E6G5O) Rabbit mAb 36778 20 µl
  • WB
  • IP
  • IF
  • F
  • ChIP
H M R 60-80 Rabbit IgG
TCF1/TCF7 (C63D9) Rabbit mAb 2203 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
H M 48, 50 Rabbit IgG
EOMES (D8D1R) Rabbit mAb 81493 20 µl
  • WB
  • IF
  • F
  • ChIP
H 75, 85 Rabbit IgG
PD-1 (D4W2J) XP® Rabbit mAb 86163 20 µl
  • WB
  • IP
  • IHC
  • F
H 52-65 Rabbit IgG
CTLA-4 (E1V6T) Rabbit mAb 96399 20 µl
  • WB
  • IP
H 25-30 Rabbit IgG
TIGIT (E5Y1W) XP® Rabbit mAb 99567 20 µl
  • WB
  • IP
  • IHC
  • IF
H 18, 30-40 Rabbit IgG
LAG3 (D2G4O™) XP® Rabbit mAb 15372 20 µl
  • WB
  • IHC
H 60-80 Rabbit IgG
TIM-3 (D5D5R™) XP® Rabbit mAb 45208 20 µl
  • WB
  • IP
  • IHC
  • F
H 45-70 Rabbit IgG
2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb 54560 20 µl
  • WB
  • IP
H M 70-120 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Human Exhausted T Cell Antibody Sampler Kit provides an economical means of characterizing the extent of exhaustion in T cells. This kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Human Exhausted T Cell Antibody Sampler Kit detects endogenous levels of its target human protein. Tox/Tox2 (E6G5O) Rabbit mAb does not cross-react with Tox3 or Tox4 proteins, but does cross-react with an unidentified protein of 50 kDa in some murine cell extracts. TCF1/TCF7 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. TIGIT (E5Y1W) XP® Rabbit mAb cross-reacts with an unidentified protein of 42 kDa in some cell extracts. 2B4/SLAMF4/CD244 (D5J9D) Rabbit mAb cross-reacts with an unidentified protein of 18 kDa in some cell extracts.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln435 of human Tox protein, Pro96 of human TCF1/TCF7 protein, Pro180 of human EOMES protein, Ala274 of human PD-1 protein, Leu95 of human CTLA-4 protein, and Asp367 of human 2B4/SLAMF4/CD244 protein. Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human TIGIT protein, the extracellular domain of human TIM-3 protein, and the amino terminus of human LAG3 protein.

Background

Tox, TCF1/TCF7, and EOMES play key roles in T cell development. Tox is also induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion. TCF1/TCF7 preserves the effector function of exhausted T cells during viral infection or cancer. EOMES is a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells. Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection, and high levels of EOMES promotes T cell exhaustion. The dynamic expression of these transcription factors help characterize the extent to which a T cell is exhausted and will respond to antigen stimulation (1-5) PD-1 (PDCD1, CD279), CTLA-4 (CD152), TIGIT (VSIG9, VSTM3), TIM-3 (HAVCR2), LAG3 (CD223), and 2B4 (SLAMF4, CD244) are immune cell co-inhibitory receptors (also known as immune checkpoints) that negatively regulate T cell function and dampen the immune response to pathogens and cancer. In addition to activated T cells, PD-1 is expressed by activated B cells and monocytes. Following interaction with its ligands, PD-L1 and PD-L2, PD-1 is phosphorylated at ITIM and ITSM motifs leading to recruitment of protein tyrosine phosphatases SHP-1 and SHP-2 and suppression of TCR signaling. CTLA-4 protein is primarily expressed on T cells, including CD8+ cytotoxic T cells, CD4+ helper T cells, and CD4+/FoxP3+ regulatory T cells. CTLA-4 protein competes with CD28 for B7.1 (CD80) and B7.2 (CD86) binding at the cell surface, resulting in downregulation of T cell activity. TIGIT is expressed at low levels on subsets of T cells and natural killer (NK) cells, and is upregulated at the protein level following activation of these cells. TIGIT marks exhausted T cells in the tumor microenvironment and during human immunodeficiency virus (HIV) infection. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer. Tumor-infiltrating macrophages and dendritic cells also express TIM-3. LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, FoxP3+ T regulatory cells (Tregs), and NK cells. 2B4 is a heterophilic cell surface receptor expressed on a variety of immune cells, including NK cells, T cells, eosinophils, mast cells, and dendritic cells. 2B4 has been shown to have both immune stimulatory and inhibitory effects on cells. Co-expression of multiple immune checkpoints help characterize the extent to which a T cell is exhausted and will respond to antigen stimulation. Therapeutic blockade of several of these immune checkpoint receptors is a promising strategy for neoplastic intervention by enabling anti-tumor immune responses (6-13).
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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