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80610
Human Reactive Exosome Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Human Reactive Exosome Marker Antibody Sampler Kit #80610

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Human Reactive Exosome Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using Syntenin-1/MDA9 (E2I9L) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of syntenin-1/MDA9 protein in Daudi cells is consistent with the predicted expression pattern.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 2
Confocal immunofluorescent analysis of SK-MEL-5 cells (left, high-expressing), A-172 cells (middle-left, high-expressing), MCF7 cells (middle-right, low-expressing), or Daudi cells (right, negative) using Syntenin-1/MDA9 (E2I9L) Rabbit mAb (green) and DAPI #4083 (blue).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from CHO-K1 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD63 protein (hCD63-Myc/DDK; +), using CD63 (E1W3T) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin Antibody #4967 (lower).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines using CD9 (D8O1A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using Flotillin-1 (D2V7J) XP® Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 6
Immunoprecipitation of syntenin-1/MDA9 protein from SK-MEL-5 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Syntenin-1/MDA9 (E2I9L) Rabbit mAb. Western blot analysis was performed using Syntenin-1/MDA9 (E2I9L) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 7
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from various cell lines using CD63 (E1W3T) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of CD63 protein in Jurkat and MOLT-4 cells is consistent with the predicted expression pattern.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 9
Western blot analysis of extracts from various cell lines using CD81 (D3N2D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal for CD81 in U-937 cells is consistent with published reports.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 11
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 12
Western blot analysis of extracts from various cell lines using TSG101 (E6V1X) Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from HCT 116 and HCT 116 Alix knockout (-/-) cells using Alix (E6P9B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 14
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD9 protein (hCD9-Myc/DDK; +), using CD9 (D8O1A) Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 15
Immunoprecipitation of flotillin-1 protein from BT-20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Flotillin-1 (D2V7J) XP® Rabbit mAb. Western blot analysis was performed using Flotillin-1 (D2V7J) XP® Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 18
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or TSG101 siRNA (+), using TSG101 (E6V1X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 19
Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using CD81 (D3N2D) Rabbit mAb.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 22
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TSG101 (hTSG101-Myc/DDK; +), using TSG101 (E6V1X) Rabbit mAb (upper), DYKDDDDK Tag (D6W5B) Rabbit mAb #14793 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 23
Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was using as a secondary antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Flotillin-1 (D2V7J) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 27
Confocal immunofluorescent analysis of HCT 116 cells (left, positive) and HCT 116 Alix knockout (-/-) cells (right, negative) using Alix (E6P9B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 30
Confocal immunofluorescent analysis of BT-20 cells using Flotillin-1 (D2V7J) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using HSP70 Antibody.
Human Reactive Exosome Marker Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded normal human liver using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 33

Small Intestine: Immunohistochemical analysis of paraffin-embedded normal human small intestine using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded normal human kidney using CD81 (D3N2D) Rabbit mAb.>
Human Reactive Exosome Marker Antibody Sampler Kit: Image 35

Immunohistochemical analysis of paraffin-embedded normal human brain using CD81 (D3N2D) Rabbit mAb.

Human Reactive Exosome Marker Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded normal human liver using CD81 (D3N2D) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Human Reactive Exosome Marker Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded U-937 cell pellet (left, positive) or RL cell pellet (right, negative) using CD81 (D3N2D) Rabbit mAb.
To Purchase # 80610
製品# サイズ 数量 価格 在庫
80610T
1 Kit  (7 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD9 (D8O1A) Rabbit mAb 13174 20 µl
  • WB
H 22, 24, 35 Rabbit IgG
CD81 (D3N2D) Rabbit mAb 56039 20 µl
  • WB
  • IHC
H 22 Rabbit IgG
CD63 (E1W3T) Rabbit mAb 52090 20 µl
  • WB
H Mk 25-60 Rabbit IgG
TSG101 (E6V1X) Rabbit mAb 72312 20 µl
  • WB
H M R 50 Rabbit IgG
Alix (E6P9B) Rabbit mAb 92880 20 µl
  • WB
  • IP
  • IF
H M R 90-100 Rabbit IgG
Flotillin-1 (D2V7J) XP® Rabbit mAb 18634 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 49 Rabbit IgG
Syntenin-1/MDA9 (E2I9L) Rabbit mAb 27964 20 µl
  • WB
  • IP
  • IF
  • F
H 30 Rabbit IgG
HSP70 Antibody 4872 20 µl
  • WB
  • IHC
H M R Mk B 72, 73 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Human Reactive Exosome Marker Antibody Sampler Kit provides an economical means of analyzing proteins that can be present on exosomes. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Human Reactive Exosome Marker Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val178 of human CD9, Ile181 of human CD81, Pro346 of human Alix, Ile368 of human flotillin-1, residues near the amino terminus of human TSG101 and human syntenin-1/MDA9, and a recombinant protein fragment of human CD63. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSP70. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Exosomes are small (30-150 nm) membrane-bound vesicles that are secreted by various cell types under normal and pathological conditions (1,2). They originate from intracellular multivesicular endosomes upon fusion with the plasma membrane. Exosomes have emerged as an important mechanism of intercellular communication facilitating the transfer of membrane and cytosolic proteins, lipids, and RNA.

A variety of methods have been described to isolate exosomes and understand their composition (3-7). Heterogeneity in exosome composition can be attributed to the cells of origin as well as the isolation methods. However, there are protein markers that appear with high frequency. Tetraspanins are a family of cell surface glycoproteins with four transmembrane domains often found in exosomes (8). Tetraspanins CD9, CD81, and CD63 appear in exosomes and have been the target of immune-affinity approaches of exosome isolation. Flotillin-1 is a lipid raft-associated integral membrane protein that is incorporated into exosomes (9). Exosomes also contain proteins involved in endosomal membrane trafficking, collectively known as the ESCRT (endosomal sorting complex required for transport) pathway. Alix regulates cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT proteins including endophilins, and CIN85 (Cbl-interacting protein of 85 kDa), and plays a role in exosome biogenesis (10-12). Syntenin-1 (MDA9, SDCBP) is a member of the PDZ family of proteins that functions as a scaffold adaptor protein regulating numerous signal transduction pathways (13). Syntenin-1 interacts with Alix to regulate exosome biogenesis (12). Tumor susceptibility gene 101 (TSG101) is a fundamental component of the ESCRT complex I involved in regulating the trafficking of proteins throughout the endosomal compartment (14). TSG101 is involved in regulating diverse biological processes, such as cell proliferation, viral budding and release, and exosome biosynthesis (15,16). The heat shock protein HSP70 is a molecular chaperone involved in protein folding that can be induced upon environmental stress (17). HSP70 may also be secreted through exosomes (18).
  1. Raposo, G. and Stoorvogel, W. (2013) J Cell Biol 200, 373-83.
  2. van Niel, G. et al. (2018) Nat Rev Mol Cell Biol 19, 213-228.
  3. Jeppesen, D.K. et al. (2019) Cell 177, 428-445.e18.
  4. Kowal, J. et al. (2016) Proc Natl Acad Sci U S A 113, E968-77.
  5. Sidhom, K. et al. (2020) Int J Mol Sci 21, 6466. doi: 10.3390/ijms21186466.
  6. Patel, G.K. et al. (2019) Sci Rep 9, 5335.
  7. Tauro, B.J. et al. (2012) Methods 56, 293-304.
  8. Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
  9. de Gassart, A. et al. (2003) Blood 102, 4336-44.
  10. Katoh, K. et al. (2003) J Biol Chem 278, 39104-13.
  11. Chatellard-Causse, C. et al. (2002) J Biol Chem 277, 29108-15.
  12. Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
  13. Pradhan, A.K. et al. (2020) Cancer Metastasis Rev 39, 769-781.
  14. Katzmann, D.J. et al. (2001) Cell 106, 145-55.
  15. Garrus, J.E. et al. (2001) Cell 107, 55-65.
  16. Zhong, Q. et al. (1998) Cancer Res 58, 2699-702.
  17. Nollen, E.A. and Morimoto, R.I. (2002) J Cell Sci 115, 2809-16.
  18. Zhan, R. et al. (2009) Biochem Biophys Res Commun 387, 229-33.

Pathways & Proteins

Explore pathways + proteins related to this product.

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