Name: Human Reactive Inflammasome Antibody Sampler Kit II
Price: 138000.00 JPY
Availability: InStock

Reviews ()

Citations (0)

Human Reactive Inflammasome Antibody Sampler Kit II: Image 1 — Simple Western™ analysis of lysates (0.1 mg/mL) from THP-1 cells using NLRP3 (D4D8T) Rabbit mAb #15101. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 2 — Western blot analysis of extracts from THP-1 and MUTZ-3 cells using NLRC4 (D5Y8E) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 3 — Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 4 — Western blot analysis of extracts from Daudi, KARPAS-299, and L-540 cell lines using AIM2 (D5X7K) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 5 — Western blot analysis of extracts from various cell lines using ASC/TMS1 (E1E3I) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Jurkat cells do not express ASC/TMS1.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 6 — Western blot analysis of extracts from mouse bone marrow-derived dendritic cells (BMDC) and various cell lines using NLRP3 (D4D8T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 7 — Western blot analysis of extracts from THP-1 and NK-92 cells using Caspase-1 (D7F10) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 8 — Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with construct overexpressing human caspase-1 (+), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb (upper) or Caspase-1 (D7F10) Rabbit mAb #3866 (lower).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 9 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 10 — Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 11 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human NLRC4 (hNLRC4; +), using NLRC4 (D5Y8E) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 12 — Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 13 — Western blot analysis of extracts from HL-60 cells, serum-starved and either untreated (-) or treated overnight with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml; +), using AIM2 (D5X7K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 14 — Immunoprecipitation of ASC/TMS1 from THP-1 cell extracts using Rabbit (D1AE) mAb XP^® Isotype Control #3900 (lane 2) or ASC/TMS1 (E1E3I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using ASC/TMS1 (E1E3I) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 15 — Immunoprecipitation of NLRP3 from J774A.1 cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or NLRP3 (D4D8T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRP3 (D4D8T) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 16 — Western blot analysis of extracts from COS cells, untransfected or transfected with human caspase-1, using Caspase-1 (D7F10) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 17 — Western blot analysis of extracts from the media of THP-1 cells, differentiated with TPA #9905 (80 nM, overnight) followed by treatment with LPS (1 ug/ml, 8 hours), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 18 — Western blot analysis of extracts from cells or media collected from THP-1 cells, differentiated with TPA #4147 (80 nM, overnight) and subsequently treated with (+) or without (-) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 19 — Immunoprecipitation of NLRC4 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or NLRC4 (D5Y8E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRC4 (D5Y8E) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 20 — Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 21 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-DDK tagged human AIM2 (hAIM2-Myc/DDK; +), using AIM2 (D5X7K) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 22 — Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using ASC/TMS1 (E1E3I) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 23 — Flow cytometric analysis of THP-1, untreated (blue, negative) or treated with LPS #14011 (100 ng/ml, 3 hr; green, positive) using IL-1β (D3U3E) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 24 — Immunoprecipitation of AIM2 from HL-60 cell extracts treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, overnight) using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or AIM2 (D5X7K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using AIM2 (D5X7K) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 25 — Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using ASC/TMS1 (E1E3I) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 26 — Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA #4174 (80 nM, 24 hr) and subsequently treated with (right) or without (left) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb (green). Actin filaments were labeled with DyLight^™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 27 — Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ASC/TMS1 (E1E3I) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (right).

Human Reactive Inflammasome Antibody Sampler Kit II: Image 28 — Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using ASC/TMS1 (E1E3I) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 29 — Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using ASC/TMS1 (E1E3I) Rabbit mAb.

Human Reactive Inflammasome Antibody Sampler Kit II: Image 30 — Immunohistochemical analysis of paraffin-embedded human spleen using ASC/TMS1 (E1E3I) Rabbit mAb.

Image Gallery

Learn more about how we get our images

To Purchase # 25620

製品#	サイズ	数量	価格	在庫
25620T	1 Kit (8 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
NLRP3 (D4D8T) Rabbit mAb 15101	20 µl	WB IP	H M	110	Rabbit IgG
AIM2 (D5X7K) Rabbit mAb 12948	20 µl	WB IP	H	40	Rabbit IgG
NLRC4 (D5Y8E) Rabbit mAb 12421	20 µl	WB IP	H	110	Rabbit IgG
Caspase-1 (D7F10) Rabbit mAb 3866	20 µl	WB IP	H	48, 20	Rabbit IgG
ASC/TMS1 (E1E3I) Rabbit mAb 13833	20 µl	WB IP IHC	H	22, 19, 15	Rabbit IgG
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 4199	20 µl	WB IP	H	20, 22	Rabbit IgG
Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb 83186	20 µl	WB IF	H	17	Rabbit IgG
IL-1β (D3U3E) Rabbit mAb 12703	20 µl	WB IF F	H	17, 31	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

3866: Western Blotting, Immunoprecipitation (Agarose)
4199: Western Blotting, Immunoprecipitation (Magnetic)
7074: Western Blotting
12421: Western Blotting, Immunoprecipitation (Agarose)
12703: Western Blotting, Immunofluorescence, Flow
12948: Western Blotting, Immunoprecipitation (Agarose)
13833: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
15101: Western Blotting, Immunoprecipitation (Agarose)
83186: Western Blotting, Immunofluorescence

Product Description

The Human Reactive Inflammasome Antibody Sampler Kit II provides an economical means of detecting multiple inflammasome components. The kit contains enough primary antibodies to perform at least two western blot experiments.

Specificity / Sensitivity

Each antibody in the Human Reactive Inflammasome Antibody Sampler Kit II detects endogenous levels of its target protein. AIM2 (D5X7K) Rabbit mAb detects a 22 kDa band of unknown origin in some cell lines. Caspase-1 (D7F10) Rabbit mAb detects endogenous levels of full-length human Caspase-1; the activated p20 subunit was detected by overexpression. ASC/TMS1 (E1E3I) Rabbit mAb can detect three known isoforms of ASC/TMS1. Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects endogenous levels of the p20 subunit of human caspase-1 only upon cleavage at Asp297. Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb recognizes endogenous levels of mature IL-1β protein only when cleaved at Asp116. IL-1β (D3U3E) Rabbit mAb is not able to detect endogenous levels of mature IL-1β. It can detect up to 100 pg of recombinant mature IL-1β.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant human IL-1β protein or with synthetic peptides corresponding to residues adjacent to Asp297 human caspase-1, residues adjacent to Asp116 of human IL-1β, residues surrounding Ala306 of mouse NLRP3, Lys93 of human AIM2, Leu942 of human NLRC4, and the carboxy terminus of human ASC/TMS1 isoform 1.

Background

The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these “danger” signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1β and IL-18 (Reviewed in 1-6). Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC/TMS1, and pro-caspase-1. The NLRP3 inflammasome is activated in a two-step process. First, NF-κB signaling is induced through PAMP- or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1β, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-β, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1β and -18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer’s disease, and autoimmune disorders.

Pathways

Explore pathways related to this product.

Database Links

UniProt ID: P01584 , Q96P20 , P29466 , O14862 , Q9NPP4 , Q9ULZ3

Entrez-Gene Id: 3553 , 114548 , 834 , 9447 , 58484 , 29108

View in PhosphoSitePlus^®

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

Human Reactive Inflammasome Antibody Sampler Kit II #25620