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93908
Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free) #93908

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  1. WB
  2. IHC
Western Blotting Image 1: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of integrin α1/CD49a protein in AsPC-1 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 2: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human invasive mammary carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human small cell carcinoma of the salivary gland using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human kidney using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human placenta (left), colon carcinoma (middle), or gastrointestinal stromal tumor (right) using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (top) or an Integrin α1/CD49a MmAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human integrin α1/CD49a. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded AsPC-1 cell pellet (left, negative), 293T cell pellet, untransfected (middle, low-expressing), and Integrin α1/CD49a-transfected (right, high-expressing) using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human neuroendocrine lung carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded normal human esophagus using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 9: Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Integrin α1/CD49a (E9K2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 93908
製品# サイズ 数量 価格 在庫
93908SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 200
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #15574. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #15574.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Integrin α1/CD49a (E9K2J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total integrin α1/CD49a protein. Non-specific staining of mucin was observed by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human integrin α1/CD49a protein.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).
Integrin α1 (CD49a, VLA-1, ITGA1) pairs with integrin β1 to form an α1β1 dimer on the cell surface. This integrin dimer plays important roles in colorectal cancer and pancreatic cancer cell proliferation, migration, and metastasis (4-6). The cytoplasmic tail of integrin α1 activates ERK and FAK/Src signaling to promote these biological processes (7,8). In immune systems, integrin α1 expression defines a tissue-resident cytotoxic T cell population contributing to both cancer immunity and vitiligo, an autoimmune disease (9,10).
  1. Liu, S. et al. (2000) J Cell Sci 113 (Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. Hogervorst, F. et al. (1993) J Cell Biol 121, 179-91.
  4. Gharibi, A. et al. (2017) Sci Rep 7, 10060.
  5. Boudjadi, S. et al. (2017) Cancers (Basel) 9, pii: E96. doi: 10.3390/cancers9080096.
  6. Shang, L. et al. (2017) Oncotarget 8, 76816-31.
  7. Abair, T.D. et al. (2008) Blood 112, 3242-54.
  8. Van Slambrouck, S. et al. (2007) Int J Oncol 31, 1501-8.
  9. Cheuk, S. et al. (2017) Immunity 46, 287-300.
  10. Molodtsov, A. and Turk, M.J. (2018) Front Immunol 9, 2810.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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For Research Use Only. Not For Use In Diagnostic Procedures.
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