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42521
LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit #42521

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LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of MEF-1 cells (left, LRP1 (+/+), positive) or PEA 13 cells (right, LRP1 (-/-), negative) using LRP1 (E2Q7S) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of Hep G2 cells (left, positive) and 293T cells (right, negative) using ApoE (pan) (D7I9N) Rabbit mAb #13366 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 3

Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of mouse striatum (left), hippocampus (middle), or midbrain (right) using Tau (D1M9X) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 5

Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Phospho-Tau (Thr181) (D9F4G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of mouse brainstem using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb #12885 (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Antibody was pre-incubated with non-phospho-Tau (Thr181) peptide (left), a phospho-Tau (Thr181) peptide (middle), or without peptide (right) to confirm phospho-specificity. Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 9

Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), or cerebellum (right) using SORL1 (D8D4G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 12

Confocal immunofluorescent analysis of SK-MEL-28 cells using Rab7 (D95F2) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of A549 cells using Rab11 (D4F5) XP® Rabbit mAb (green). Actin filaments were labeled wtih DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 14

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 15

Western blot analysis of extracts from various cell lines and tissues using LRP1 (E2Q7S) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human small intestine using ApoE (pan) (D7I9N) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 17

Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 18

Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 19

Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb. The phospho-specificity of Phospho-Tau (Thr181) (D9F4G) Rabbit mAb was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Thr181.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 20

Confocal immunofluorescent analysis of mouse primary neurons using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (green). Blue pseudocolor = Hoescht 33342 #4082 (fluorescent DNA dye).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 21

Western blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 22

Western blot analysis of extracts from various tissues using SORL1 (D8D4G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 23

Western blot analysis of extracts from various cell lines using Rab5 (C8B1) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 24

Western blot analysis of extracts from various cell lines using Rab7 (D95F2) XP® Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 25

Western blot analysis of extracts from various cell lines using Rab11 (D4F5) XP® Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human kidney using ApoE (pan) (D7I9N) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 29

Western blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded human skin using ApoE (pan) (D7I9N) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human spleen using ApoE (pan) (D7I9N) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 35

Immunohistochemical analysis of paraffin-embedded mouse colon, control (left) or λ phosphatase-treated (right), using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 36

Immunoprecipitation of ApoE from Hep G2 cells treated with Brefeldin A #9972 (10 ng/ml, 90 min), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ApoE (pan) (D7I9N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ApoE (pan) (D7I9N) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 37

Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 38

Western blot analysis of cell extracts from Hep G2 cells treated with Brefeldin A #9972 (10 ng/ml, 90 min) and human cerebellum using ApoE (pan) (D7I9N) Rabbit mAb.

LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit: Image 39

Western blot analysis of cell extracts from 293T cells, treated with Brefeldin A #9972 (10 ng/ml, 90 min; +) and mock transfected (-) or transfected with a construct expressing full-length human ApoE2 (hApoE2; +), ApoE3 (hApoE3; +), or ApoE4 (hApoE4; +), using ApoE (pan) (D7I9N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

To Purchase # 42521T
製品番号 サイズ 価格 在庫
42521T
1 Kit  (9 x 20 µl)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
LRP1 (E2Q7S) Rabbit mAb 26387 20 µl
  • WB
  • IF
H M R 85 Rabbit IgG
ApoE (pan) (D7I9N) Rabbit mAb 13366 20 µl
  • WB
  • IP
  • IHC
  • IF
H 35 Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl
  • WB
  • IHC
  • IF
H M R 50-80 Rabbit IgG
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb 12885 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 50-80 Rabbit IgG
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) 20194 20 µl
  • WB
  • IP
  • IF
H M R 50-80 Rabbit IgG
SORL1 (D8D4G) Rabbit mAb 79322 20 µl
  • WB
  • IF
H M 250 Rabbit IgG
Rab5 (C8B1) Rabbit mAb 3547 20 µl
  • WB
  • IF
H M R Mk 25 Rabbit 
Rab7 (D95F2) XP® Rabbit mAb 9367 20 µl
  • WB
  • IP
  • IF
H M R Mk 23 Rabbit IgG
Rab11 (D4F5) XP® Rabbit mAb 5589 20 µl
  • WB
  • IP
  • IF
H M R Mk 25 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit provides an economical means of detecting components of the LRP-1 mediated intercellular transmission of human tau using antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit detects endogenous levels of its target protein. LRP1 (E2Q7S) Rabbit mAb recognizes endogenous levels of total LRP1 β subunit. ApoE (pan) (D7I9N) Rabbit mAb recognizes overexpressed ApoE2, ApoE3, and ApoE4 proteins. Phospho-Tau (Thr181) (D9F4G) Rabbit mAb recognizes endogenous levels of tau protein only when phosphorylated at Thr181. Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) recognizes endogenous levels of tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Ser404 or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403, and dual phosphorylation at Ser400/Thr403. Rab11 (D4F5) XP® Rabbit mAb detects endogenous levels of Rab11 protein including isoforms Rab11a and Rab11b.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu4488 of human LRP1 protein, Pro285 of human ApoE protein, Asp430 of human tau protein, Glu267 of human SORL1 protein, Gly190 of human Rab5A protein, and Glu188 of human Rab7 protein, respectively. Rab11 (D4F5) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Rab11 protein. Phospho-Tau (Thr181) (D9F4G) Rabbit mAb and Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr181 of human Tau protein and Ser400/Thr403/Ser404 of human Tau protein, respectively.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. In addition to its normal function, intracellular neurofibrillary tangle protein aggregates, composed of hyperphosphorylated helical bundles of tau, are a major hallmark of neurodegenerative diseases like Alzheimer's disease (AD) (1). Moreover, disease progression is also measured by the progressive spread and deposition of the protein aggregates via intercellular transfer of tau (2). Although the intercellular mechanism of protein aggregate transfer is poorly understood, low density lipoprotein receptor related protein 1 (LRP1) was identified as a regulator of tau uptake and spread (3). LRP1 is a type I transmembrane receptor that mediates the endocytosis of various ligands, including apolipoproteins and tau. Interestingly, human apolipoprotein E (ApoE), which also binds to LRP1, is genetically linked to AD (4). LRP1-mediated protein uptake, in addition to tau, may play an important role in AD progression. In addition to LRP1, other low density lipoprotein receptor related proteins, including SORL1, are genetically linked to AD, suggesting a conserved cellular mechanism that converges on this family of proteins that contributes to AD etiology (5). Once tau binds to LRP1, receiving cells are likely to internalize and process tau via the endosomal pathway, completing cell-to-cell transmission. Rab5, Rab7, and Rab11, members of the Ras superfamily of small Rab GTPases, are likely to regulate endosomal processing of tau (6). 

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Braak, H. and Braak, E. (1991) Acta Neuropathol 82, 239-59.
  3. Rauch, J.N. et al. (2020) Nature 580, 381-385.
  4. Corder, E.H. et al. (1993) Science 261, 921-3.
  5. Yin, R.H. et al. (2015) Mol Neurobiol 51, 909-18.
  6. Zhang, X. et al. (2019) ACS Chem Neurosci 10, 828-838.

Pathways & Proteins

Explore pathways + proteins related to this product.

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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