|MW (kDa)||125, 142|
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly508 of mouse MAP1S protein.
MAP1S is a member of the microtubule-associated protein (MAP) family. There are at least ten proteins in the MAP family, including MAP1A, MAP1B, MAP1S, MAP2, MAP6, MAP7, MAP9, Tau, DCLK, and DCX. MAP1 subfamily members express predominantly in brain cells regulating several physiological processes in cells, including cytoskeleton regulation that, in some cases are energy-dependent, including the autophosphorylation and phosphorylation of other MAP family members, and other cytoskeletal proteins (1,2). MAP1 family members have been identified as high-molecular-weight proteins consisting of the full-length (FL), at least one heavy chain (HC), one light chain (LC), and, in some members short chains (SC) (2,3). The FL-MAP1S is formed by the HC and LC, forming the active MAP1S complex. The LC binds, bundles, and stabilizes microtubules, and the HC regulates LC activity (2). MAP1S also interacts with Nemitin (neuronal enriched MAP interacting protein), enhancing cytoskeleton regulation (4).
MAP1S contributes to mitochondria stabilization by interacting with mitochondrion-associated leucine-rich PPR-motif containing protein (LRPPRC). MAP1S regulates ubiquitination processes like autophagosome biogenesis and protein clearance. These processes are MAP1S isoform-specific (5). An increased level of MAP1S enhances autophagy to remove p62-associated aggresomes and dysfunctional organelles that trigger DNA double-strand breaks and genome instability (6).
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.