Name: Microglia Cross Module Antibody Sampler Kit
Price: 148000.00 JPY
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Microglia Cross Module Antibody Sampler Kit: Image 1 — Flow cytometric analysis of Daudi cells (blue) and MJ cells (green) using Lamin A/C (4C11) Mouse mAb (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4408 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 2 — Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using Lamin A/C (4C11) Mouse mAb #4777. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Microglia Cross Module Antibody Sampler Kit: Image 3 — Flow cytometric analysis of K-562 cells using Stat2 (D9J7L) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 4 — Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using Axl (C89E7) Rabbit mAb #8661. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Microglia Cross Module Antibody Sampler Kit: Image 5 — Western blot analysis of extracts from various cell lines using IQGAP1 (D8K4X) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 6 — Western blot analysis of extracts from HeLa cells, treated with either 10 μM of MG132 (to accumulate hydroxylated HIF-1α) or 10 µM MG132 and 1 mM DMOG (to accumulate nonhyroxylated HIF-1α), using Hydroxy-HIF-1α (Pro564) (D43B5) XP^® Rabbit mAb (upper) or total HIF-1α Antibody #3716 (lower).

Microglia Cross Module Antibody Sampler Kit: Image 7 — Western blot analysis of cell extracts from Baf3, 32D, and mouse spleen using HS1 (D5A9) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame truncation mutation in the gene encoding LMNA (lane 2) using Lamin A/C (4C11) Mouse mAb #4777 (upper) or α-actinin (D6F6) XP^® Rabbit mAb #6487 (lower). The change in LMNA molecular weight in the mutated HeLa cells is consistent with an in-frame deletion.

Microglia Cross Module Antibody Sampler Kit: Image 9 — Western blot analysis of extracts from J774A.1 and Raw 264.7 cells using ASC/TMS1 (D2W8U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Microglia Cross Module Antibody Sampler Kit: Image 10 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Microglia Cross Module Antibody Sampler Kit: Image 11 — Western blot analysis of extracts from various cell lines using Stat2 (D9J7L) Rabbit mAb. KARPAS cell line source: Dr Abraham Karpas at the University of Cambridge.

Microglia Cross Module Antibody Sampler Kit: Image 12 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and Stat2 (D9J7L) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across GAS5 gene.

Microglia Cross Module Antibody Sampler Kit: Image 13 — Western blot analysis of HeLa Cell Extracts, untreated (-) or Axl knock-out (+) using Axl (C89E7) Rabbit mAb, #8661 (upper) or GAPDH (D16H11) XP^® Rabbit mAb, #5174 (lower).

Microglia Cross Module Antibody Sampler Kit: Image 14 — Western blot analysis of extracts from serum-starved U266 and A-431 cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml; +) using Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb (upper) and total Stat2 (D9J7L) Rabbit mAb #72604 (lower).

Microglia Cross Module Antibody Sampler Kit: Image 15 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) (10nM, 30min) and Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across GATAD2B, a known target gene of Stat2 (see additional figure containing CUT&RUN-qPCR data).

Microglia Cross Module Antibody Sampler Kit: Image 16 — Confocal immunofluorescent analysis of the ventricular zone in P21 mouse brain using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DyLight^™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 17 — breast:
Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using IQGAP1 (D8K4X) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 18 — Confocal immunofluorescent analysis of HeLa cells, treated with either 10 μM MG132 (left) or 10 μM MG132 and 1 mM DMOG (right), using Hydroxy-HIF-1α (Pro564) (D43B5) XP^® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red).

Microglia Cross Module Antibody Sampler Kit: Image 19 — Immunohistochemical analysis of paraffin-embedded mouse spleen using HS1 (D5A9) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 20 — Western blot analysis of extracts from various cell lines using Lamin A/C (4C11) Mouse mAb.

Microglia Cross Module Antibody Sampler Kit: Image 21 — Immunoprecipitation of ASC/TMS1 from J774A.1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is ASC (D2W8U) Rabbit mAb. Western blot analysis was performed using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 22 — Immunoprecipitation of Stat2 from KARPAS-299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is Stat2 (D9J7L) Rabbit mAb. Western blot was performed using Stat2 (D9J7L) Rabbit mAb. KARPAS cell line source: Dr Abraham Karpas at the University of Cambridge.

Microglia Cross Module Antibody Sampler Kit: Image 23 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and Stat2 (D9J7L) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 1 (upper), including GAS5 gene (lower).

Microglia Cross Module Antibody Sampler Kit: Image 24 — Western blot analysis of extracts from various cell lines using Axl (C89E7) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Microglia Cross Module Antibody Sampler Kit: Image 25 — Confocal immunofluorescent analysis of A-431 cells, serum starved (left) or treated with IFNα (1000 U/ml for 30 min; right) using Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb (green) and β-actin (8H10D10) Mouse mAb #3700 (red).

Microglia Cross Module Antibody Sampler Kit: Image 26 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) (10nM, 30min) and Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across RFX5 (upper) and GATAD2B (lower), a known target gene of Stat2 (see additional figure containing CUT&RUN-qPCR data).

Microglia Cross Module Antibody Sampler Kit: Image 27 — Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 28 — Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and LNCaP (right) cell pellets using IQGAP1 (D8K4X) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 29 — Immunohistochemical analysis of paraffin-embedded LL2 syngeneic tumor using HS1 (D5A9) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 30 — Western blot analysis of extracts from THP-1 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hr), using Lamin A/C (4C11) Mouse mAb.

Microglia Cross Module Antibody Sampler Kit: Image 31 — Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or RAW 264.7 cell pellet (right, negative) using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 32 — Confocal immunofluorescent analysis of A-431 cells, serum starved (left) or treated with IFNα (1000 U/ml for 30 mins; right) using Stat2 (D9J7L) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 33 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and either Stat2 (D9J7L) Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human SIN3A promoter primers and human ITM2A upstream primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Microglia Cross Module Antibody Sampler Kit: Image 34 — Immunohistochemical analysis of paraffin-embedded breast carcinoma using Axl (C89E7) Rabbit mAb. Note staining of infiltrating cells.

Microglia Cross Module Antibody Sampler Kit: Image 35 — Flow cytometric analysis of U266 cells, untreated (blue) or treated with IFN-α (green) using Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')₂ fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 36 — CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) (10nM, 30min) and either Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human GATAD2B promoter primers and human C22orf34 downstream primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Microglia Cross Module Antibody Sampler Kit: Image 37 — Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 38 — Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IQGAP1 (D8K4X) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 39 — Confocal immunofluorescent analysis of mouse Tg2576 brain which overexpresses mutant human APP695. Sections were first labeled with HS1 (D5A9) XP^® Rabbit mAb (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). After blocking free secondary binding sites with Mouse (G3A1) mAb IgG1 Isotype Control #5415, sections were incubated with GFAP (GA5) Mouse mAb (Alexa Fluor^® 647 Conjugate) #3657 (red). Nuclei were labeled with Hoechst 33342 #4082 (blue).

Microglia Cross Module Antibody Sampler Kit: Image 40 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Lamin A/C (4C11) Mouse mAb.

Microglia Cross Module Antibody Sampler Kit: Image 41 — Immunohistochemical analysis of paraffin-embedded mouse forestomach using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 42 — Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Axl (C89E7) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 43 — Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IQGAP1 (D8K4X) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 44 — Confocal immunofluorescent analysis of 32D cells (left) and C2C12 cells (right), using HS1 (D5A9) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 45 — Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Lamin A/C (4C11) Mouse mAb.

Microglia Cross Module Antibody Sampler Kit: Image 46 — Immunohistochemical analysis of paraffin-embedded mouse brain using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 47 — Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (10nM) for 30 min and Stat2 (D9J7L) Rabbit mAb, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across USP18, a known target gene of Stat2 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.

Microglia Cross Module Antibody Sampler Kit: Image 48 — Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Axl (C89E7) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 49 — Confocal immunofluorescent analysis of A549 (left) and Hep G2 (right) cells using IQGAP1 (D8K4X) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 50 — Flow cytometric analysis of NIH/3T3 cells (red) and 32D cells (blue), using HS1 (D5A9) XP^® Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 51 — Immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (green) and MAP2 Antibody #4542 (red).

Microglia Cross Module Antibody Sampler Kit: Image 52 — Immunohistochemical analysis of paraffin-embedded mouse colon using ASC/TMS1 (D2W8U) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (right).

Microglia Cross Module Antibody Sampler Kit: Image 53 — Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (10nM) for 30 min and Stat2 (D9J7L) Rabbit mAb, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 22 (upper), including USP18 (lower), a known target gene of Stat2 (see additional figure containing ChIP-qPCR data).

Microglia Cross Module Antibody Sampler Kit: Image 54 — Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Axl (C89E7) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 55 — Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Microglia Cross Module Antibody Sampler Kit: Image 56 — Immunohistochemical analysis of paraffin-embedded mouse thymus using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 57 — Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (100 ng/ml) for 30 min, and either Stat2 (D9J7L) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human USP18 promoter primers, SimpleChIP^® Human WARS Intron 1 Primers #30101, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Microglia Cross Module Antibody Sampler Kit: Image 58 — Immunohistochemical analysis of paraffin-embedded metastatic lung carcinoma using Axl (C89E7) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 59 — Flow cytometric analysis of HeLa cells (green) using Lamin A/C (4C11) Mouse mAb (solid lines) or a concentration matched Mouse (G3A1) mAb IgG Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4408 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 60 — Immunohistochemical analysis of paraffin-embedded mouse small intestine using ASC/TMS1 (D2W8U) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 61 — Immunohistochemical analysis of paraffin-embedded cell pellets, NCI-H1299 (left) or Jurkat (right), using Axl (C89E7) Rabbit mAb.

Microglia Cross Module Antibody Sampler Kit: Image 62 — Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor (top left), 4T1 syngeneic mammary tumor (top right), Renca cell pellet (bottom left), and 4T1 cell pellet (bottom right) using ASC/TMS1 (D2W8U) Rabbit mAb. Both tumors show staining of infiltrating immune cells. Note the presence of staining in the Renca tumor cells and the lack of staining in the 4T1 tumor cells consistent with staining results on corresponding cell pellets.

Microglia Cross Module Antibody Sampler Kit: Image 63 — Confocal immunofluorescent analysis of DU 145 (left) and HCC827 (right) cells using Axl (C89E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microglia Cross Module Antibody Sampler Kit: Image 64 — Flow cytometric analysis of fixed and permeabilized Jurkat cells (blue, negative) and DU145 cells (green, positive) using Axl (C89E7H4) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Microglia Cross Module Antibody Sampler Kit: Image 65 — Confocal immunofluorescent analysis of mouse Tg2576 brain which overexpresses mutant human APP695. Sections were first labeled with ASC/TMS1 (D2W8U) Rabbit mAb #67824 (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). After blocking free secondary binding sites with Mouse (G3A1) mAb IgG1 Isotype Control #5415, sections were incubated with GFAP (GA5) Mouse mAb (Alexa Fluor^® 647 Conjugate) #3657 (red). Nuclei were labeled with Hoechst 33342 #4082 (blue).

Microglia Cross Module Antibody Sampler Kit: Image 66 — Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) either untreated (upper left) or treated with LPS (50 ng/ml, 4 hr, middle) or LPS followed by ATP (5 mM, 45 min, upper right), and J774A.1 (lower left) or Raw 264.7 (lower right) cells, using ASC/TMS1 (D2W8U) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and ATP (white arrows).

Microglia Cross Module Antibody Sampler Kit: Image 67 — Flow cytometric analysis of Raw264.7 cells (blue) and J774A.1 cells (green) using ASC/TMS1 (D2W8U) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

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To Purchase # 83163

製品#	サイズ	数量	価格	在庫
83163T	1 Kit (9 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
ASC/TMS1 (D2W8U) Rabbit mAb 67824	20 µl	WB IP IHC IF F	M	22	Rabbit IgG
HS1 (D5A9) XP^® Rabbit mAb 3892	20 µl	WB IP IHC IF F	M R	80	Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129	20 µl	IF F	H M R	359	Rabbit IgG
Axl (C89E7) Rabbit mAb 8661	20 µl	WB IP IHC IF F	H Mk	138	Rabbit IgG
Hydroxy-HIF-1α (Pro564) (D43B5) XP^® Rabbit mAb 3434	20 µl	WB IP IF	H Mk	120	Rabbit IgG
Stat2 (D9J7L) Rabbit mAb 72604	20 µl	WB IP IF F ChIP C&R	H M	97, 113	Rabbit IgG
Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb 88410	20 µl	WB IF F C&R	H R	97, 113	Rabbit IgG
Lamin A/C (4C11) Mouse mAb 4777	20 µl	WB IP IHC IF F	H M R Mk	74 (Lamin A), 63 (Lamin C)	Mouse IgG2a
IQGAP1 (D8K4X) XP^® Rabbit mAb 20648	20 µl	WB IHC IF	H M R Mk	195	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

3434: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
3892: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
4777: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
7074: Western Blotting
8661: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
9129: Immunofluorescence, Immunofluorescence, Flow
20648: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence
67824: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
72604: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence*, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
88410: Western Blotting, Immunofluorescence*, Flow, CUT&RUN Assay

Product Description

The Microglia Cross Module Antibody Sampler Kit provides an economical means of detecting proteins identified as markers of microglial activity corresponding to proliferation, neurodegeneration, interferon and LPS-relation by western blot and/or immunofluorescence.

Specificity / Sensitivity

Each antibody in the Microglia Cross Module Antibody Sampler Kit detects endogenous levels of its target protein. Hydroxy-HIF-1α (Pro564) (D43B5) XP^® Rabbit mAb detects endogenous levels of HIF-1α only when hydroxylated at Pro564. This antibody may cross-react with other overexpressed proline hydroxylated proteins. Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb recognizes endogenous levels of Stat2 protein only when phosphorylated at Tyr690. Axl (C89E7) Rabbit mAb does not cross-react with Tyro3. HS1 (D5A9) XP^® Rabbit mAb (Rodent Specific) does not recognize human HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels. Lamin A/C (4C11) Mouse mAb detects endogenous levels of lamin A and lamin C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu310 of mouse HS1, Pro564 of human HIF-1α, Leu706 of human Stat2, Tyr690 of human Stat2, the amino terminus of human Ki-67 and IQGAP1, and recombinant fragment of human Axl, human lamin A, and mouse ASC/TMS1.

Background

Distinct microglial activation states have been identified using RNA-seq data from a vast array of neurological disease and aging models. These activation states have been categorized into modules corresponding to proliferation, neurodegeneration, interferon-relation, LPS-relation, and many others (1). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (2). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (3) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (4).
Ki-67 is a nuclear nonhistone protein (5) universally expressed among proliferating cells and absent in quiescent cells (6). Axl is a receptor tyrosine kinase that binds Gas6, stimulating regulatory effects on microglial phagocytic response to inflammatory stimuli (7). Hypoxia inducible factor-1 (HIF-1α) is a transcription factor responsible for adaptation to low oxygen environments whose downstream effects have been shown in a number of neurodegenerative diseases. Under normoxic conditions, HIF-1α is proline hydroxylated leading to ubiquitin mediated degradation (8). Stat2 is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (9,10). In response to IFN-alpha/beta, Stat2 is activated by phosphorylation at site Tyr690 through associations with receptor-bound Jak kinases (11). Lamins are nuclear membrane structural components important for maintaining normal cell functions. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. The cleavage of lamins results in nuclear dysregulation and cell death (12,13). IQGAP1 is ubiquitously expressed and has been found to interact with APC (14) and the CLIP170 complex in response to small GTPases, promoting cell polarization and migration (15).

Pathways

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Database Links

UniProt ID: P52630 , Q16665 , P30530 , P46940 , P46013 , P14317 , Q9EPB4 , P02545

Entrez-Gene Id: 6773 , 3091 , 558 , 8826 , 4288 , 3059 , 66824 , 4000

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Limited Uses

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Microglia Cross Module Antibody Sampler Kit #83163