Cat. # | Size | Qty. | Price | Inventory |
---|---|---|---|---|
92654S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 55,62 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His422 of human NOTUM protein.
NOTUM is an extracellular carboxylesterase and a key negative regulator of the Wnt signaling pathway. The palmitoleoylation of Wnt is required for its binding to Frizzled receptors, which is essential for Wnt signaling activation. NOTUM removes the palmitoleate moiety on Wnt ligands to inactivate Wnt signaling (1,2). The function of NOTUM is dependent on glypicans, which bind both NOTUM and Wnt to co-localize them at the cell surface. NOTUM is expressed in the liver (3), bone (4), epithelial cells in the intestine (5), and brain (6) to regulate tissue formation and function. Biological aging may result from increased NOTUM expression, while inhibition of NOTUM may rejuvenate stem cells to promote tissue regeneration (5). In colorectal cancer and gastric cancer, aberrant high NOTUM expression promotes proliferation and metastasis. Knockdown or inhibition of NOTUM activity suppresses its cancer-promoting effects (7-9). Inhibition of NOTUM activity may represent a new approach to treat disease where aberrant NOTUM activity has been identified as the underlying cause (10).
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