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42801
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free) #42801

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 42801
製品# サイズ 数量 価格 在庫
42801SF
100 µg

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 280
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #11818. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #11818

Storage

Store at -20°C.This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Pathways & Proteins

Explore pathways + proteins related to this product.

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For Research Use Only. Not For Use In Diagnostic Procedures.
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