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61381
PICALM Signaling Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

PICALM Signaling Antibody Sampler Kit #61381

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Western blot analysis of extracts from various cell lines and mouse brain tissue using Cathepsin D (E7Z4L) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of fixed frozen wild-type mouse brain using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (magenta pseudocolor).
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (magenta pseudocolor).
Confocal immunofluorescent analysis of fixed frozen wild-type mouse brain (left) or brain from an amyloid mouse model of Alzheimer's disease (right) using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red), GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (cyan pseudocolor), and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (blue pseudocolor).
Projected confocal z-stack of fixed frozen mouse kidney using Cathepsin D (E7Z4L) XP® Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #20825 (green) and DAPI #4083 (blue).
Confocal immunofluorescent analysis of NIH/3T3 cells using Cathepsin D (E7Z4L) XP® Rabbit mAb (green) and DAPI #4083 (blue).
Western blot analysis of extracts from various cell lines using PICALM (E3J9R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.
Confocal immunofluorescent tile scan of a fixed frozen brain from an amyloid mouse model of Alzheimer's disease using EEA1 (C45B10) Rabbit mAb #3288 (green), GFAP (GA5) Mouse mAb #3670 (red), and DAPI #4083 (blue).
Confocal immunofluorescent analysis of fixed frozen mouse thalamus, labeled with EEA1 (C45B10) Rabbit mAb #3288 (left, green) and co-labeled with GFAP (GA5) Mouse mAb #3670 (right, red) and DAPI #4083 (right, blue).
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus, labeled with EEA1 (C45B10) Rabbit mAb #3288 (left, green) and co-labeled with GFAP (GA5) Mouse mAb #3670 (right, red) and DAPI #4093 (right, blue).
Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunohistochemical analysis of paraffin-embedded mouse liver using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.
Western blot analysis of extracts from control HCT 116 cells (lane 1) or PICALM knockdown (KD) HCT 116 cells (lane 2) using PICALM (E3J9R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).
Confocal immunofluorescent analysis of SH-SY5Y cells using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse kidney using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP® Rabbit mAb.
Immunoprecipitation of PICALM protein from U-118 MG extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and lane 3 is PICALM (E3J9R) Rabbit mAb. Western blot analysis was performed using PICALM (E3J9R) Rabbit mAb. Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb #93702 was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded mouse lung using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP® Rabbit mAb.
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum, labeled with PICALM (E3J9R) Rabbit mAb (green) and DAPI #4083 (blue).
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP® Rabbit mAb.
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer’s disease labeled with PICALM (E3J9R) Rabbit mAb (left, red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (right, green).
Immunohistochemical analysis of paraffin-embedded mouse thymus using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of fixed frozen mouse kidney, labeled with PICALM (E3J9R) Rabbit mAb (green) and DAPI #4083 (blue).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Cathepsin D (E7Z4L) XP® Rabbit mAb.

Flow cytometric analysis of Jurkat cells using LAMP1 (D2D11) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of fixed frozen mouse liver, labeled with PICALM (E3J9R) Rabbit mAb (green) and DAPI #4083 (blue).
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HCT 116 cells, either mock transfected (left, high-expressing) or transfected with siRNA directed against PICALM (right, low-expressing), using PICALM (E3J9R) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunohistochemical analysis of paraffin-embedded mouse colon using Cathepsin D (E7Z4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
To Purchase # 61381
Cat. # Size Qty. Price Inventory
61381T
1 Kit  (5 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PICALM (E3J9R) Rabbit mAb 26765 20 µl
  • WB
  • IP
  • IF
H M R 68, 70 Rabbit IgG
Clathrin Heavy Chain (D3C6) XP® Rabbit mAb 4796 20 µl
  • WB
  • IP
  • IF
H M R Mk 190 Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb 9091 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 20 µl
  • WB
  • IP
  • IF
H M R 170 Rabbit IgG
Cathepsin D (E7Z4L) XP® Rabbit mAb 88239 20 µl
  • WB
  • IHC
  • IF
M 46, 43, 28 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Product Description

The PICALM Signaling Antibody Sampler Kit provides an economical means of investigating PICALM signaling by western blot and labeling endo-lysosomal components by immunofluorescence (IF). This kit includes enough primary antibodies to perform two western blot experiments or at least 40 IF tests per primary antibody.

Background

The antibodies in this kit serve to characterize phosphatidylinositol-binding clathrin assembly protein (PICALM)-mediated lysosomal maturation, as endo-lysosomal systems are important for normal physiology and prevention of common late-onset neurodegenerative diseases such as Alzheimer's disease (AD).

PICALM is a clathrin-binding protein involved in the endo-lysosomal pathway, where it has been genetically associated with AD (1,2). Clathrin is a triskelion-shaped protein that plays a major role in the formation of coated vesicles (1). Formation of these vesicles is critical for shaping the cell membrane to promote intracellular trafficking for multiple membrane trafficking pathways in the cell, including the trans-Golgi network as well as transport to and from the cell membrane and endosomal compartments. PICALM disruption increases the number of early endosomes, which is linked to exacerbated tau aggregation (2).  Early endosome antigen 1 (EEA1) is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (3,4). Lysosome-associated membrane protein 1 (LAMP1) is an abundant lysosomal membrane protein involved in regulating lysosomal motility during lysosome-phagosome fusion (5,6). Cathepsin D (CSTD) is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (7). Mutations in PICALM were shown to cause lysosomal enzymes and membrane proteins to be mis-trafficked and accumulated; for example, immature forms of CTSD accumulate abnormally within endosomes. These changes correlate with reduced turnover of lysosomal cargoes generated by autophagy and endocytosis (2).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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