The Plasma Membrane Marker Antibody Sampler Kit provides an economical means of detecting plasma membrane markers in various cell types. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Specificity / Sensitivity
Each antibody in the Plasma Membrane Marker Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb recognizes endogenous levels of Na,K-ATPase α1 protein only when phosphorylated at Tyr10, and cross-reacts with an induced 75-80 kDa doublet of unknown origin. Pan-Cadherin (28E12) Rabbit mAb detects endogenous levels of total cadherin proteins. 4F2hc/CD98 (D3F9D) XP® Rabbit mAb recognizes endogenous levels of total 4F2hc/CD98 protein, and is predicted to detect multiple isoforms of 4F2hc/CD98.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro12 of human Na,K-ATPase α1, Pro780 of human E-cadherin, Arg526 of human N-cadherin, Val509 of human 4F2hc/CD98, Glu20 of human caveolin-1, Leu520 of human ENPP1, a conserved region of human N-, R-, E- and P-cadherin proteins, or a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of human Na,K-ATPase α1 protein.
The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. Phosphorylation of Na,K-ATPase at Tyr10 has been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters (1). Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells (2). 4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters, such as LAT1 and LAT2, and regulates uptake of amino acids (3). The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions (4). Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. ENPP1 plays important roles in bone mineralization and soft tissue calcification (5).