Name: Polycomb Group 2 (PRC2) Antibody Sampler Kit
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Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 1 — Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and SUZ12 (D39F6) XP^® Rabbit mAb using a protocol based on the RBP-eCLIP Kit from EclipseBio. The figure shows binding across the NOTCH1 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 2 — Western blot analysis of extracts from F9 cells and NTERA-2 cells, untreated (-) or treated with retinoic acid (1 μM, 5 d; +) to induce differentiation, using JARID2 (D6M9X) Rabbit mAb (upper) and GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower). As expected, JARID2 protein levels decrease upon NTERA-2 cell differentiation.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 3 — CUT&RUN was performed with NCCIT cells and JARID2 (D6M9X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across HoxA, a known target gene of JARID2 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 4 — Western blot analysis of extracts from various cell lines using AEBP2 (D7C6X) Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 5 — Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 6 — Western blot analysis of extracts from various cell lines using EZH1 (D7D5D) Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 7 — Western blot analysis of extracts from 293T cells, either transfected with nontargeting siRNA (293T siNT) or siRNA targeting EED (293T siEED), and F9 cells using EED Antibody (upper) and GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 9 — Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Ezh2 (D2C9) XP^® Rabbit mAb performed on the Leica BOND Rx.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 10 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 11 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either JARID2 (D6M9X) Rabbit mAb, CBX8 (D2O8C) Rabbit mAb #14696, or AEBP2 (D7C6X) Rabbit mAb #14129, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. All of JARID2, CBX8, and AEBP2 are known to associate with PRC complexes. The figure shows binding of JARID2, CBX8, and AEBP2 across HOXD gene region, known target genes of JARID2, CBX8, and AEBP2 (see additional figure containing ChIP-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 12 — CUT&RUN was performed with NCCIT cells and JARID2 (D6M9X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA (upper) and HoxD (lower), known target genes of JARID2 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 13 — Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® AEBP2 siRNA I #14711 (+), or SignalSilence^® AEBP2 siRNA II #14713 (+), using AEBP2 (D7C6X) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower). The AEBP2 (D7C6X) Rabbit mAb confirms silencing of AEBP2 expression, while the α-Actinin (D6F6) XP^® Rabbit mAb is used as a loading control.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 14 — Confocal immunofluorescent analysis of HeLa cells (left) and NTERA2 cells (right) using SUZ12 (D39F6) XP^® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 15 — Western blot analysis of extracts from EZH1 wild-type (WT) and knockout (KO) HCT 116 cells and other cell lines using EZH1 (D7D5D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, the EZH1 knockout HCT 116 cells do not express EZH1 protein.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 16 — Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Ezh2 (D2C9) XP^® Rabbit mAb performed on the Leica BOND Rx.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 17 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either JARID2 (D6M9X) Rabbit mAb, CBX8 (D2O8C) Rabbit mAb #14696, or AEBP2 (D7C6X) Rabbit mAb #14129, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. All of JARID2, CBX8, and AEBP2 are known to associate with PRC complexes. The figure shows binding of JARID2, CBX8, and AEBP2 across HOXA (upper) and HOXD (lower), known target genes of JARID2, CBX8, and AEBP2 (see additional figure containing ChIP-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 18 — CUT&RUN was performed with NCCIT cells and either JARID2 (D6M9X) Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 19 — Immunoprecipitation of AEBP2 from NCCIT cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or AEBP2 (D7C6X) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AEBP2 (D7C6X) Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 20 — Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SUZ12 (D39F6) XP^® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 21 — Immunoprecipitation of EZH1 from NCCIT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is EZH1 (D7D5D) Rabbit mAb. Western blot analysis was performed using EZH1 (D7D5D) Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 22 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 23 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either JARID2 (D6M9X) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 24 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either JARID2 (D6M9X) Rabbit mAb #13594, CBX8 (D2O8C) Rabbit mAb #14696, or AEBP2 (D7C6X) Rabbit mAb, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP^® ChIP-seq DNA Library Prep Kit for Illumina^® #56795. All of JARID2, CBX8, and AEBP2 are known to associate with PRC complexes. The figure shows binding of JARID2, CBX8, and AEBP2 across HOXA gene region, known target genes of JARID2, CBX8, and AEBP2 (see additional figure containing ChIP-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 25 — Flow cytometric analysis of HeLa cells using SUZ12 (D39F6) XP^® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 647 Conjugate) #4414 was used as a secondary antibody.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 26 — Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 27 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either JARID2 (D6M9X) Rabbit mAb #13594, CBX8 (D2O8C) Rabbit mAb #14696, or AEBP2 (D7C6X) Rabbit mAb, using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP^® ChIP-seq DNA Library Prep Kit for Illumina^® #56795. All of JARID2, CBX8, and AEBP2 are known to associate with PRC complexes. The figure shows binding of JARID2, CBX8, and AEBP2 across HOXA (upper) and HOXD (lower), known target genes of JARID2, CBX8, and AEBP2 (see additional figure containing ChIP-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 28 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP^® Rabbit mAb or RING1B (D22F2) XP^® Rabbit mAb, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. SUZ12 and RING1B are known to associate with each other on chromatin. The figure shows binding of both SUZ12 and RING1B across HOXD genes, which are known target genes of both SUZ12 and RING1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 29 — Immunohistochemical analysis of paraffin-embedded human lymphoma using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 30 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either AEBP2 (D7C6X) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 31 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP^® Rabbit mAb or RING1B (D22F2) XP^® Rabbit mAb, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. SUZ12 and RING1B are known to associate with each other on chromatin. The figure shows binding of both SUZ12 and RING1B across HOXA genes (upper) and HOXD genes (lower), which are known target genes of SUZ12 (see additional figure containing ChIP-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 32 — Immunohistochemical analysis of paraffin-embedded 4T1 mouse syngeneic tumor using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 33 — Confocal immunofluorescent analysis of mouse hippocampus (left) and cerebellum (right) using Ezh2 (D2C9) XP^® Rabbit mAb (green). Actin filaments were labeled with DyLight^™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 34 — CUT&RUN was performed with NCCIT cells and AEBP2 (D7C6X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across FZD5 gene, a known target gene of AEBP2 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 35 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP^® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 36 — Confocal immunofluorescent analysis of HeLa cells using Ezh2 (D2C9) XP^® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (blue). Actin filaments were labeled with DY-554 phalloidin (red).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 37 — Immunohistochemical analysis of paraffin-embedded mouse brain using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 38 — CUT&RUN was performed with NCCIT cells and AEBP2 (D7C6X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 2 (upper), including FZD5 gene (lower), a known target gene of antibody’s short name (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 39 — CUT&RUN was performed with NCCIT cells and SUZ12 (D39F6) XP^® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA1, a known target gene of SUZ12 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 40 — Flow cytometric analysis of human peripheral blood mononuclear cells untreated (left) and treated (right) with anti-human CD3 (10ug/ml, coated plates) and anti-human CD28 (5ug/ml) for 3 days at 37ºC using EZH2 (D2C9) XP^® Rabbit mAb and co-stained with an anti-human CD3 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 41 — Immunohistochemical analysis of paraffin-embedded mouse liver using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 42 — CUT&RUN was performed with NCCIT cells and treatment and either AEBP2 (D7C6X) Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human SOX2 promoter primers, human FZD5 promoter primers, and human DTX2 promoter primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 43 — CUT&RUN was performed with NCCIT cells and SUZ12 (D39F6) XP^® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxD (upper) and HoxA (lower), a known target gene of SUZ12 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 44 — Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP^® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across the MYT1 gene. For additional ChIP-seq tracks, please download the product datasheet.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 45 — Immunohistochemical analysis of paraffin-embedded mouse testis using Ezh2 (D2C9) XP^® Rabbit mAb.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 46 — CUT&RUN was performed with NCCIT cells and either SUZ12 (D39F6) XP^® Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 47 — Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP^® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across chromosome 20 (upper), including MYT1 gene (lower).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 48 — Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Ezh2 (D2C9) XP^® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 49 — CUT&RUN was performed with NCCIT cells and Ezh2 (D2C9) XP^® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA1, a known target gene of Ezh2 (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 50 — CUT&RUN was performed with NCCIT cells and Ezh2 (D2C9) XP^® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina^® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 7 (upper), including HoxA genes (lower), a known cluster of EZH2 target genes (see additional figure containing CUT&RUN-qPCR data).

Polycomb Group 2 (PRC2) Antibody Sampler Kit: Image 51 — CUT&RUN was performed with NCCIT cells and either Ezh2 (D2C9) XP^® Rabbit mAb or Rabbit (DA1E) mAb IgG XP^® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HoxA1 Intron 1 Primers #7707, SimpleChIP^® Human HoxA2 Promoter Primers #5517, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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To Purchase # 62083

製品#	サイズ	数量	価格	在庫
62083T	1 Kit (6 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Ezh2 (D2C9) XP^® Rabbit mAb 5246	20 µl	WB IP IHC IF F ChIP C&R	H M R Mk	98	Rabbit IgG
EZH1 (D7D5D) Rabbit mAb 42088	20 µl	WB IP	H Mk	95	Rabbit IgG
SUZ12 (D39F6) XP^® Rabbit mAb 3737	20 µl	WB IP IF F ChIP C&R eCLIP	H M R Mk	83	Rabbit IgG
EED Antibody 51673	20 µl	WB	H M R Mk	50-70	Rabbit
JARID2 (D6M9X) Rabbit mAb 13594	20 µl	WB IP ChIP C&R	H M	150	Rabbit IgG
AEBP2 (D7C6X) Rabbit mAb 14129	20 µl	WB IP ChIP C&R	H M R Mk	70, 28	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

Product Description

The Polycomb Group 2 (PRC2) Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group 2 Proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.

Specificity / Sensitivity

Each antibody in the Polycomb Group 2 (PRC2) Antibody Sampler Kit detects endogenous levels of target proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Arg354 of human Ezh2, Pro257 of human SUZ12 protein, Leu23 of human EED, Asp1114 of human JARID2, Leu345 of human AEBP2, and the carboxy terminus of EZH1.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of multiple developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1-4). Enhancer of zest homolog 1 (Ezh1) and enhancer of zest homolog 2 (Ezh2) are members of this large protein family and are subunits of the polycomb repressor complex 2 (PRC2), also known to contain SUZ12, EED, JARID2, and AEBP2. Ezh1 and its paralog Ezh2 are mutually exclusive catalytic subunits of the PRC2 complex, which functions to mono-, di-, and tri-methylate Lys27 on histone H3, all marks that are associated with transcriptional repression. SUZ12 and EED proteins are also absolutely required for methyltransferase activity (5). JARID2 and AEBP2 are both accessory proteins that function to recruit the PRC2 complex to target genes and enhance methyltransferase activity by binding to DNA and histone proteins in nucleosomes (5-13).

Pathways

Explore pathways related to this product.

Database Links

UniProt ID: Q15022 , Q92833 , O75530 , Q6ZN18 , Q92800 , Q15910

Entrez-Gene Id: 23512 , 3720 , 8726 , 121536 , 2145 , 2146

View in PhosphoSitePlus^®

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Polycomb Group 2 (PRC2) Antibody Sampler Kit #62083