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83308
TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free) #83308

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  1. IHC
Immunohistochemistry Image 1: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TMEM119 (E3E4T) Mouse mAb (left) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lymphoma using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human cerebellum (left) or colon carcinoma (right) using TMEM119 (E3E4T) Mouse mAb (top) or TMEM119 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human TMEM119. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or TMEM119-transfected (right), using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human brain using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human testis using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human lung using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using TMEM119 (E3E4T) Mouse mAb. Data were generated using the standard formulation of this product.
To Purchase # 83308
製品# サイズ 数量 価格 在庫
83308SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG2a

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #44134. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #44134.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TMEM119 (E3E4T) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total TMEM119 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM119 protein.

Background

Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). These DAM microglia are conserved in human and mouse and have been identified in Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP/PS1 mouse models (3,4). This protein’s specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cells types in neurodegenerative disease models (1,2).
  1. Bennett, M.L. et al. (2016) Proc Natl Acad Sci U S A 113, E1738-46.
  2. Satoh, J. et al. (2016) Neuropathology 36, 39-49.
  3. Deczkowska, A. et al. (2018) Cell 173, 1073-81.
  4. Keren-Shaul, H. et al. (2017) Cell 169, 1276-1290.e17.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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For Research Use Only. Not For Use In Diagnostic Procedures.
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