注目情報はこちら >>
61129
TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free) #61129

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
Western Blotting Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human TRAC/TCRα constant protein (hTRAC/TCRα constant-Myc/DDK), Myc/DDK-tagged full-length human TRBC1/TCRβ constant region 1 protein (hTRBC1/TCRβ constant region 1-Myc/DDK), Myc/DDK-tagged full-length human TRBC2/TCRβ constant region 2 protein (hTRBC2/TCRβ constant region 2-Myc/DDK), Myc/DDK-tagged full-length human TRDC/TCRδ constant protein (hTRDC/TCRδ constant-Myc/DDK), and Myc/DDK-tagged full-length human TRGC1/TCRγ constant region 1 protein (hTRGC1/TCRγ constant region 1-Myc/DDK), using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Show More
Western Blotting Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from PEER and Jurkat cell lines using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human thymus using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded PEER cell pellet (left, positive) or Raji cell pellet (right, negative) using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Show More
Immunohistochemistry Image 5: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 6: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 7: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human spleen using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

To Purchase # 61129
製品# サイズ 数量 価格 在庫
61129SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY
MW (kDa) 40-45
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #55750. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #55750.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total TRDC/TCRδ protein. This antibody does not cross-react with human TRAC/TCRα constant, TRBC1/TCRβ constant region 1, TRBC2/TCRβ constant region 2, or TRGC1/TCRγ constant region 1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein.

Background

The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4).
  1. Brenner, M.B. et al. Nature 322, 145-9.
  2. Davis, M.M. and Bjorkman, P.J. (1988) Nature 334, 395-402.
  3. Vantourout, P. and Hayday, A. (2013) Nat Rev Immunol 13, 88-100.
  4. Kabelitz, D. et al. (2020) Cell Mol Immunol 17, 925-939.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Powered by Translations.com GlobalLink OneLink SoftwarePowered By OneLink