Name: Lung Cancer RTK Antibody Sampler Kit
Price: 182000.00 JPY
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Lung Cancer RTK Antibody Sampler Kit: Image 1 — Simple Western™ analysis of lysates (1.0 mg/mL) from HCC-78 cells using ROS1 (D4D6® ) Rabbit mAb #3287. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.

Lung Cancer RTK Antibody Sampler Kit: Image 2 — Simple Western™ analysis of lysates (0.1 mg/mL) from A-431 cells using EGF Receptor (D38B1) XP^® Rabbit mAb #4267. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.

Lung Cancer RTK Antibody Sampler Kit: Image 3 — Simple Western™ analysis of lysates (0.1 mg/mL) from SK-BR-3 cells using HER2/ErbB2 (D8F12) XP^® Rabbit mAb #4290. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Lung Cancer RTK Antibody Sampler Kit: Image 4 — Western blot analysis of extracts from various cell lines using Ret (E1N8X) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 5 — Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb (upper), and total EGFR control antibody (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 6 — Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (L858R) protein using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper), and total EGFR control antibody (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 7 — Western blot analysis of extracts from HCC78 (SLC34A2-ROS1), U-118 MG (FIG-ROS1), and HeLa (ROS1 negative) cells using ROS1 (D4D6^®) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Note: HCC78 cells express the 85, 70, and 59 kDa forms of the SLC34A2-ROS1 fusion protein (7).

Lung Cancer RTK Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from NCI-H2228 and NCI-H3122 cells using ALK (D5F3^®) XP^® Rabbit mAb. Variants denoting fusions of different EML4 exons (v1 or v3) are indicated.

Lung Cancer RTK Antibody Sampler Kit: Image 9 — Western blot analysis of extracts from control Hela cells (lane 1), or EGFR knockout Hela cells (lane 2) using EGF Receptor (D38B1) XP^® Rabbit mAb #4267, (upper) or #8457 β-Actin (D6A8) Rabbit mAb (lower). The absence of signal in EGFR-knockout Hela cells confirms specificity of the antibody for EGFR.

Lung Cancer RTK Antibody Sampler Kit: Image 10 — Western blot analysis of extracts from SK-BR-3 and MCF7 cells using HER2/ErbB2 (D8F12) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 11 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Lung Cancer RTK Antibody Sampler Kit: Image 12 — Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP^® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 13 — Western blot analysis of extracts from A-204 (FGFR1 positive), KG-1a (FGFR1 oncogenic partner-FGFR1 fusion), A172 (FGFR1 low), and HT-29 (FGFR1 negative) cells using FGF Receptor 1 (D8E4) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 14 — Immunohistochemical analysis of paraffin-embedded human adrenal gland using Ret (E1N8X) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 15 — Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb (upper) and total EGFR (D38B1) XP^® Rabbit mAb #4267 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 16 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing wildtype ROS1 (hROS1; +), using ROS1 (D4D6^®) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 17 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma with high (left) and low levels (right) of ALK expression using ALK (D5F3^®) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 18 — Western blot analysis of extracts from A-431, BxPC3 and HeLa cells using EGF Receptor (D38B1) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 19 — Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb performed on the Leica^® BOND^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 20 — Western blot analysis of extracts from control HeLa cells (lane 1) or Met knockout HeLa cells (lane 2) using Met (D1C2) XP^® Rabbit mAb #8198. The absence of signal in the Met knockout HeLa cells confirms specificity of the antibody for Met.

Lung Cancer RTK Antibody Sampler Kit: Image 21 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 1 (D8E4) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 22 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ret (E1N8X) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 23 — Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb (upper) and total EGFR (D38B1) XP^® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).

Lung Cancer RTK Antibody Sampler Kit: Image 24 — Immunoprecipitation of ROS1 from HCC78 cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or ROS1 (D4D6^®) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ROS1 (D4D6^®) Rabbit mAb, followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076.

Lung Cancer RTK Antibody Sampler Kit: Image 25 — Immunohistochemical analysis of paraffin-embedded anaplastic large cell lymphoma (ALCL) using ALK (D5F3^®) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 26 — Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb performed on the Leica^® BOND^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 27 — Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Met (D1C2) XP^® Rabbit mAb performed on the Leica^® Bond^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 28 — Immunohistochemical analysis of paraffin-embedded human kidney using FGF Receptor 1 (D8E4) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 29 — Immunohistochemical analysis of paraffin-embedded human small intestine using Ret (E1N8X) XP^® Rabbit mAb. Note the positive signal of the myenteric plexus in the muscularis externa of the small intestine.

Lung Cancer RTK Antibody Sampler Kit: Image 30 — Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenograft (right, EGFR deletion positive) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP^® Rabbit mAb #4267 (lower).

Lung Cancer RTK Antibody Sampler Kit: Image 31 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ROS1 (D4D6^®) Rabbit mAb. Note: Staining is of FIG-ROS1 fusion (6).

Lung Cancer RTK Antibody Sampler Kit: Image 32 — Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using ALK (D5F3^®) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 33 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGF Receptor (D38B1) Rabbit mAb performed on the Leica^® BOND^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 34 — Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb performed on the Leica^® BOND^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 35 — Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Met (D1C2) XP^® Rabbit mAb performed on the Leica^® Bond^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 36 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 1 (D8E4) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 37 — Immunohistochemical analysis of paraffin-embedded TT (left) or Hep G2 (right) cell pellets using Ret (E1N8X) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 38 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma of known mutational status using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP^® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).

Lung Cancer RTK Antibody Sampler Kit: Image 39 — Immunohistochemical analysis of paraffin-embedded human lung carcinomas showing distinct localization using ROS1 (D4D6^®) Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 40 — Immunohistochemical analysis of paraffin-embedded MDA-MB-468 (amplified EGFR, left), HT-29 (low EGFR, middle) and CAMA-1 (EGFR negative, right) cells using EGF Receptor (D38B1) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 41 — Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb performed on the Leica^® BOND^™ Rx.

Lung Cancer RTK Antibody Sampler Kit: Image 42 — Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 43 — Confocal immunofluorescent analysis of TT (positive, left) and HeLa (negative, right) cells using Ret (E1N8X) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 44 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ROS1 (D4D6^®) Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 45 — Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using EGF Receptor (D38B1) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 46 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 47 — Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Met (D1C2) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 48 — Confocal immunofluorescent analysis of A204 cells (positive, left), KG-1 cells (positive, middle) and A172 cells (weak expression, right) using FGF Receptor 1 (D8E4) XP^® Rabbit mAb (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 49 — Flow cytometric analysis of fixed and permeabilized HeLa cells (blue, negative) and TT cells (green, positive) using Ret (E1N8X) XP^® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 50 — Immunohistochemical analysis of paraffin-embedded HCC78 xenograft using ROS1 (D4D6^®) Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 51 — Flow cytometric analysis of U-937 cells (blue) and KARPAS 299 cells (green) using ALK (D5F3^®) XP^® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 52 — Immunohistochemical analysis of paraffin-embedded human placenta using EGF Receptor (D38B1) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 53 — Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using HER2/ErbB2 (D8F12) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 54 — Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 55 — Flow cytometric analysis of A-204 cells using FGF Receptor 1 (D8E4) XP^® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 56 — Confocal immunofluorescent analysis of HCC78 (left) or HeLa (right) cells using ROS1 (D4D6^®) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 57 — Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGF Receptor (D38B1) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 58 — Immunohistochemical analysis of paraffin-embedded SK-BR-3 (Her2 high, left) and MCF7 cell pellets (Her2 low, right) using HER2/ErbB2 (D8F12) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 59 — Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 60 — Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb (upper, green) and EGFR (D38B1) XP^® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 61 — Flow cytometric analysis of HeLa cells (blue, negative) and HCC78 cells (green, positive) using ROS1 (D4D6^®) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 62 — Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with human epidermal growth factor (right), using EGF Receptor (D38B1) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 63 — Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 64 — Flow cytometric analysis of Jurkat cells (blue) and A431 cells (green) using EGF Receptor (D38B1) XP^® Rabbit mAb #4267 (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 65 — Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 66 — Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper, green) and EGFR (D38B1) XP^® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 67 — Flow cytometric analysis of fixed and permeabilized Ramos cells (blue, negative) and MKN-45 cells (green, positive) using Met (D1C2) XP^® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Lung Cancer RTK Antibody Sampler Kit: Image 68 — Flow cytometric analysis of fixed and permeabilized Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb.

Lung Cancer RTK Antibody Sampler Kit: Image 69 — Confocal immunofluorescent analysis of H3255 (left), HCC827 (middle) and A431 cells (right) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). H3255 cells have the L858R EGFR mutation, HCC827 cells have an exon 19 EGFR deletion, and A431 cells overexpress wild type EGFR. Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Lung Cancer RTK Antibody Sampler Kit: Image 70 — Flow cytometric analysis of two non-small cell lung cancer cell lines using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb. H3255 cells (green) harbor the L858R EGFR mutation while the H1650 cells (blue) have the exon 19 mutation (delE746-A750).

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To Purchase # 79884

製品#	サイズ	数量	価格	在庫
79884T	1 Kit (9 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
EGF Receptor (D38B1) XP^® Rabbit mAb 4267	20 µl	WB IP IHC IF F	H M Mk	175	Rabbit IgG
EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb 3197	20 µl	WB IP IHC IF F	H	175	Rabbit IgG
EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb 2085	20 µl	WB IP IHC IF F	H	175	Rabbit IgG
ALK (D5F3^®) XP^® Rabbit mAb 3633	20 µl	WB IP IHC F	H	220 (ALK), 80 (NPM-ALK), 117 (EML4-ALK v1), 86 (EML4-ALK v3)	Rabbit IgG
HER2/ErbB2 (D8F12) XP^® Rabbit mAb 4290	20 µl	WB IHC	H M	185	Rabbit IgG
FGF Receptor 1 (D8E4) XP^® Rabbit mAb 9740	20 µl	WB IP IHC IF F	H M R Mk	92 , 120, 145	Rabbit IgG
ROS1 (D4D6^®) Rabbit mAb 3287	20 µl	WB IP IHC IF F	H	258, 110, 50-80	Rabbit IgG
Ret (E1N8X) XP^® Rabbit mAb 14556	20 µl	WB IHC IF F	H M	150, 175	Rabbit IgG
Met (D1C2) XP^® Rabbit mAb 8198	20 µl	WB IP IHC IF F	H	140, 170	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

2085: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
3197: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
3287: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
3633: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Flow
4267: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
4290: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin)
7074: Western Blotting
8198: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
9740: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
14556: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow

Product Description

The Lung Cancer RTK Antibody Sampler Kit provides an economical means of detecting receptor tyrosine kinases (RTKs) associated with lung cancer. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Lung Cancer RTK Antibody Sampler Kit detects endogenous levels of its target protein. FGF Receptor 1 (D8E4) XP^® Rabbit mAb may slightly cross-react with overexpressed FGF receptor family members. IHC staining for ROS1 (D4D6^®) Rabbit mAb may be observed in ROS1 rearranged lung carcinomas, macrophages/giant cells, reactive type II pneumocyte hyperplasia, and the epithelium in areas of bronchiolar metaplasia. Staining of unknown specificity has been observed in cholangiocarcinoma, hepatocellular carcinoma, and kidney tissues. HER2/ErbB2 (D8F12) XP^® Rabbit mAb may cross-react slightly with other overexpressed RTKs. EGF Receptor (D38B1) XP^® Rabbit mAb does not cross-react with other proteins of the ErbB family. Species cross-reactivity for IHC-P, IHC-BOND, and IF-IC is human only. EGFR (L858R Mutant Specific) (43B2) Rabbit mAb may cross-react with wild-type EGFR and other HER family members when highly overexpressed. Careful titration of this antibody may be required to obtain optimal specificity.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding E746-A750del and L865 mutant sequences of human EGFR, Pro320 of human Ret protein, and residues near the carboxy terminus of human Met protein, or recombinant proteins specific to the carboxy terminus of human FGF receptor 1, human ALK, human ROS1, the amino terminus of human HER2/ErbB2, and the cytoplasmic domain of human EGF receptor.

Background

Lung cancer is the leading cause of cancer-related mortality worldwide (1). It is generally divided into two broad histological classifications: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC comprises about 80-85% of all lung cancers. Receptor tyrosine kinases (RTKs) are essential components to cellular signaling pathways and are often overexpressed or otherwise dysregulated by genetic mutations, fusion, or gene amplification (2,3). RTKs are generally activated by receptor specific ligands, leading to autophosphorylation and the subsequent recruitment of downstream signaling proteins. The most common RTK amplification in NSCLC is that for epidermal growth factor receptor (EGFR). Also, two of the most common mutations in EGFR include an exon 19 deletion, E746-A750, and a point mutation L858R (4,5). In addition to EGFR, several other RTKs may become aberrantly activated in NSCLC, including ALK, ROS1, HER2/ErbB2, Met, Ret, FGF Receptor 1, and NTRK (6). Specific tyrosine kinase inhibitors (TKIs) have been part of the arsenal of treating the disease and so analyzing the expression and mutational status of these receptors plays an important role in personalized treatment.

Pathways

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Database Links

UniProt ID: P08922 , Q9UM73 , P00533 , P08581 , P11362 , P04626 , P07949

Entrez-Gene Id: 6098 , 238 , 1956 , 4233 , 2260 , 2064 , 5979

View in PhosphoSitePlus^®

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Lung Cancer RTK Antibody Sampler Kit #79884