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26768
Parthanatos Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Parthanatos Antibody Sampler Kit #26768

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Parthanatos Antibody Sampler Kit: Image 1
Confocal immunofluorescent analysis of HeLa cells either untreated (left), treated with hydrogen peroxide (500 mM, 5 min; center), or treated with PARP inhibitor Talazoparib (100 nmol/L, 3 hr) followed by hydrogen peroxide (500 mM, 5 min; right) using Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb #83732 (green), S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red), and DAPI #4083 (blue).
Parthanatos Antibody Sampler Kit: Image 2
Simple Western™ analysis of lysates (1 mg/mL) from Jurkat cells treated with Etoposide (25 uM, 5 hours) using PARP (46D11) Rabbit mAb #9532. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Parthanatos Antibody Sampler Kit: Image 3
Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb.
Parthanatos Antibody Sampler Kit: Image 4
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Parthanatos Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using MIF (E8S8H) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of MIF protein in SNB-19 cells is consistent with mRNA expression profiles reported in public bioinformatic databases, confirming specificity of the antibody for MIF.
Parthanatos Antibody Sampler Kit: Image 6
Western blot analysis of Colo 205 cells treated (+) with combinations of the following treatments as indicated: hydrogen peroxide (500 μM, 5 min), hydrogen peroxide-treated lysates treated with phosphodiesterase 1 (0.5 μg/mL, 4 hr at 37ºC), or with tcPARG (5 μM, 4 hr at 37ºC ) using Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb (upper), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Parthanatos Antibody Sampler Kit: Image 7
Western blot analysis of extracts from control HEK293 cells (lane 1) or PARP knockout HEK293 cells (lane 2) using PARP (46D11) Rabbit mAb #9532 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the PARP knockout HEK293 cells confirms the specificity of the antibody for PARP.
Parthanatos Antibody Sampler Kit: Image 8
Confocal analysis of mouse small intestine using AIF (D39D2) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Parthanatos Antibody Sampler Kit: Image 9
Confocal immunofluorescent analysis of mouse testis (left) and skeletal muscle (right) using MIF (E8S8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Parthanatos Antibody Sampler Kit: Image 10
Western blot analysis of Colo 205 cells untreated (-), or treated with hydrogen peroxide (500 μM, 5 min; +), using Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb (upper), or β-Tubulin (9F3) Rabbit mAb #2128 (lower).
Parthanatos Antibody Sampler Kit: Image 11
Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.
Parthanatos Antibody Sampler Kit: Image 12
Confocal immunofluorescent analysis of mouse brainstem using AIF (D39D2) XP® Rabbit mAb #5318 (green) and GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Parthanatos Antibody Sampler Kit: Image 13
Confocal immunofluorescent analysis of U-87 MG cells (left, positive) or SNB-19 cells (right, negative) using MIF (E8S8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Parthanatos Antibody Sampler Kit: Image 14
Confocal immunofluorescent analysis of mouse cerebellum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
Parthanatos Antibody Sampler Kit: Image 15
Confocal immunofluorescent analysis of mouse striatum using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red), and F4/80 (BM8.1) Rat mAb #71299 (blue).
Parthanatos Antibody Sampler Kit: Image 16
Confocal immunofluorescent analysis of mouse thalamus using AIF (D39D2) XP® Rabbit mAb #5318 (green), GFAP (GA5) Mouse mAb #3670 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Parthanatos Antibody Sampler Kit: Image 17
Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Parthanatos Antibody Sampler Kit: Image 18
Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP ™ Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
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To Purchase # 26768
製品# サイズ 数量 価格 在庫
26768T		 1 Kit  (4 x 20 microliters)

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PARP (46D11) Rabbit mAb 9532 20 µl
  • WB
  • IP
 H M R Mk 116, 89 Rabbit 
AIF (D39D2) XP® Rabbit mAb 5318 20 µl
  • WB
  • IP
  • IF
 H M R Mk 67 Rabbit IgG
MIF (E8S8H) Rabbit mAb 75038 20 µl
  • WB
  • IF
 H M R Hm 12 Rabbit IgG
Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb 83732 20 µl
  • WB
  • IF
 H All Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Parthanatos Antibody Sampler Kit provides an economical means of detecting the activation of parthanatos. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Parthanatos Antibody Sampler Kit detects endogenous levels of its target protein. PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 or PARP-3. Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb recognizes endogenous levels of ADP-ribosylated proteins and does not cross-react with other post-translational modifications.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Gly623 of human PARP-1, Ala520 of human AIF, and Tyr100 of human MIF protein, or with KLH modified on lysines with ADP ribose.

Background

Parthanatos is a form of regulated cell death that follows a multistep cascade and is triggered by the accumulation of poly (ADP-ribose) (PAR). When PAR polymerase-1 (PARP-1) is overactivated under certain conditions, excessive PAR is produced and binds to apoptosis-inducing factor (AIF). As a result, AIF is released from the mitochondria and forms a complex with macrophage migration inhibitory factor (MIF). Subsequently, the AIF/MIF complex is translocated to the nucleus where MIF cleaves genomic DNA into large fragments, and cell death follows (1-3). Studies have found that parthanatos is involved in the pathogenesis of many diseases, particularly neurodegenerative disorders, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS) (4-7).
  1. Fatokun, A.A. et al. (2014) Br J Pharmacol 171, 2000-16.
  2. Wang, Y. et al. (2016) Science 354, aad6872. doi: 10.1126/science.aad6872.
  3. Liu, L. et al. (2022) Cell Mol Life Sci 79, 60.
  4. Park, H. et al. (2020) Int Rev Cell Mol Biol 353, 1-29.
  5. Wang, X. and Ge, P. (2020) Neuroscience 449, 241-250.
  6. Zhou, Y. et al. (2021) Pharmacol Res 163, 105299.
  7. Thapa, K. et al. (2021) Life Sci 267, 118975.

Pathways & Proteins

Explore pathways + proteins related to this product.

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