Name: Fanconi Anemia Antibody Sampler Kit
Price: 107000.00 JPY
Availability: InStock

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Fanconi Anemia Antibody Sampler Kit: Image 1 — Western blot analysis of extracts from various cell lines using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower).

Fanconi Anemia Antibody Sampler Kit: Image 2 — Western blot analysis of extracts from various cell types using FANCB (D9W6S) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 3 — Western blot analysis of extracts from various cell lines using FANCA (D1L2Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower). As expected, GM06914 cells are negative for FANCA.

Fanconi Anemia Antibody Sampler Kit: Image 4 — Western blot analysis of extracts from HeLa, U-2 OS, and GM16633 (PD20, FANCD2-negative) cells using FANCD2 (D5L5X) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower).

Fanconi Anemia Antibody Sampler Kit: Image 5 — Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.

Fanconi Anemia Antibody Sampler Kit: Image 6 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Fanconi Anemia Antibody Sampler Kit: Image 7 — Western blot analysis of DLD1 cells, either wild type (WT) or BRCA2 knockout (-/-), using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb (lower). DLD1 (WT) and DLD1 (-/-) cells were kindly provided by Dr. Judit Jimenez, Yale University, New Haven, CT.

Fanconi Anemia Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FANCB (FANCB-Myc/DDK; +) protein, using FANCB (D9W6S) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 9 — Immunoprecipitation of FANCA from 293T cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or FANCA (D1L2Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using FANCA (D1L2Z) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 10 — Western blot analysis of extracts from HeLa cells, untreated or treated with mitomycin C (2 μg/mL, 24 hr), using FANCD2 (D5L5X) Rabbit mAb. DNA damage caused by mitomycin C induces monoubiquitination of FANCD2, altering its electrophoretic mobility and increasing its apparent molecular weight (4).

Fanconi Anemia Antibody Sampler Kit: Image 11 — Immunoprecipitation of FANCD2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP^® Isotype Control #3900, and lane 3 is FANCD2 (D5L5X) Rabbit mAb. Western blot analysis was performed using FANCD2 (D5L5X) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 12 — Confocal immunofluorescent analysis of U-2 OS cells (left; FANCD2-positive) and GM16633 cells (right; PD20, FANCD2-negative) using FANCD2 (D5L5X) Rabbit mAb #16323 (green). Actin filaments were labeled with DyLight^™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong^® Gold Antifade Reagent with DAPI #8961 (blue).

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To Purchase # 74437

製品#	サイズ	数量	価格	在庫
74437T	1 Kit (5 x 20 microliters)

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
FANCA (D1L2Z) Rabbit mAb 14657	20 µl	WB IP	H	160	Rabbit IgG
FANCB (D9W6S) Rabbit mAb 14243	20 µl	WB	H M R	90	Rabbit IgG
FANCD2 (D5L5X) Rabbit mAb 16323	20 µl	WB IP IF	H	155, 162	Rabbit IgG
BRCA2 (D9S6V) Rabbit mAb 10741	20 µl	WB	H	380	Rabbit IgG
BACH1/BRIP1 Antibody 4578	20 µl	WB	H	145	Rabbit
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

4578: Western Blotting
7074: Western Blotting
10741: Western Blotting
14243: Western Blotting
14657: Western Blotting, Immunoprecipitation (Magnetic)
16323: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence

Product Description

The Fanconi Anemia Antibody Sampler Kit provides an economical means of detecting members of the Fanconi Anemia signaling pathway. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Fanconi Anemia Antibody Sampler kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ala514 of human FANCA, Pro280 of human FANCB, Gly995 of human FANCD2, and a recombinant protein specific to the carboxy terminus of human BRCA2. Polyclonal BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Background

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1. FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are also recruited to sites of DNA damage (3).

Pathways

Explore pathways related to this product.

Database Links

UniProt ID: Q9BXW9 , Q9BX63 , P51587 , Q8NB91 , O15360

Entrez-Gene Id: 2177 , 83990 , 675 , 2187 , 2175

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Limited Uses

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Fanconi Anemia Antibody Sampler Kit #74437