注目情報はこちら >>
96128
Mouse Reactive Exosome Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
  1. Products
  2. Primary Antibodies
  3. Mouse Reactive Exosome Marker Antibody Sampler Kit

Mouse Reactive Exosome Marker Antibody Sampler Kit #96128

Reviews ()
Citations (0)
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using CD81 (D5O2Q) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using Flotillin-1 (D2V7J) XP® Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 4
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using TSG101 (E6V1X) Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from HCT 116 and HCT 116 Alix knockout (-/-) cells using Alix (E6P9B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 7
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD9 protein (mCD9-Myc/DDK; +), using CD9 (E8L5J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD81 (mCD81-Myc/DDK, +), using CD81 (D5O2Q) Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 9
Immunoprecipitation of flotillin-1 protein from BT-20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Flotillin-1 (D2V7J) XP® Rabbit mAb. Western blot analysis was performed using Flotillin-1 (D2V7J) XP® Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 11
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or TSG101 siRNA (+), using TSG101 (E6V1X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 12
Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from various cell lines using CD9 (E8L5J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Absence of signal in BA/F3 cells is predicted from RNAseq data and confirms the specificity of the antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 14
Immunoprecipitation of CD81 from extracts of C2C12 cells. Lane 1 is 10% input, Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD81 (D5O2Q) Rabbit mAb. Western blot analysis was performed using CD81 (D5O2Q) Rabbit mAb.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 15
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 16
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TSG101 (hTSG101-Myc/DDK; +), using TSG101 (E6V1X) Rabbit mAb (upper), DYKDDDDK Tag (D6W5B) Rabbit mAb #14793 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 17
Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was using as a secondary antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 18
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) or BA/F3 cells (right, negative) using CD9 (E8L5J) Rabbit mAb (green) and DAPI #4083 (blue).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 19
Confocal immunofluorescent analysis of C2C12 (positive, left) and BaF3 (negative, right) cells using CD81 (D5O2Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Flotillin-1 (D2V7J) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 22
Confocal immunofluorescent analysis of HCT 116 cells (left, positive) and HCT 116 Alix knockout (-/-) cells (right, negative) using Alix (E6P9B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 23
Flow cytometric analysis of live BA/F3 cells (blue, negative) and JAWSII cells (green, positive) using CD9 (E8L5J) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 25
Flow cytometric analysis of fixed and permeabilized BA/F3 cells (blue, negative) and JAWSII cells (green, positive) using CD9 (E8L5J) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 26
Confocal immunofluorescent analysis of BT-20 cells using Flotillin-1 (D2V7J) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using HSP70 Antibody.
Mouse Reactive Exosome Marker Antibody Sampler Kit: Image 28
Flow cytometric analysis of live EL4 cells (blue, negative) and A20 cells (green, positive) using CD81 (D5O2Q) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Image Gallery
Learn more about how we get our images
To Purchase # 96128
製品# サイズ 数量 価格 在庫
96128T		 1 Kit  (6 x 20 microliters)

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD9 (E8L5J) Rabbit mAb 98327 20 µl
  • WB
  • IF
  • F
 M R 22-27 Rabbit IgG
CD81 (D5O2Q) Rabbit mAb 10037 20 µl
  • WB
  • IP
  • IF
  • F
 M 22 Rabbit IgG
TSG101 (E6V1X) Rabbit mAb 72312 20 µl
  • WB
 H M R 50 Rabbit IgG
Alix (E6P9B) Rabbit mAb 92880 20 µl
  • WB
  • IP
  • IF
 H M R 90-100 Rabbit IgG
Flotillin-1 (D2V7J) XP® Rabbit mAb 18634 20 µl
  • WB
  • IP
  • IHC
  • IF
 H M R 49 Rabbit IgG
HSP70 Antibody 4872 20 µl
  • WB
  • IHC
 H M R Mk B 72, 73 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Mouse Reactive Exosome Marker Antibody Sampler Kit provides an economical means of analyzing proteins that can be present on exosomes. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Mouse Reactive Exosome Marker Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu119 of mouse CD9, Pro184 of mouse CD81, Pro346 of human Alix, Ile368 of human flotillin-1, and residues near the amino terminus of human TSG101. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSP70. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Exosomes are small (30-150 nm) membrane-bound vesicles that are secreted by various cell types under normal and pathological conditions (1,2). They originate from intracellular multivesicular endosomes upon fusion with the plasma membrane. Exosomes have emerged as an important mechanism of intercellular communication facilitating the transfer of membrane and cytosolic proteins, lipids, and RNA.

A variety of methods have been described to isolate exosomes and understand their composition (3-7). Heterogeneity in exosome composition can be attributed to the cells of origin as well as the isolation methods. However, there are protein markers that appear with high frequency. Tetraspanins are a family of cell surface glycoproteins with four transmembrane domains often found in exosomes (8). Tetraspanins CD9, CD81, and CD63 appear in exosomes and have been the target of immune-affinity approaches of exosome isolation. Flotillin-1 is a lipid raft-associated integral membrane protein that is incorporated into exosomes (9). Exosomes also contain proteins involved in endosomal membrane trafficking, collectively known as the ESCRT (endosomal sorting complex required for transport) pathway. Alix regulates cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT proteins including endophilins, and CIN85 (Cbl-interacting protein of 85 kDa), and plays a role in exosome biogenesis (10-12). Syntenin-1 (MDA9, SDCBP) is a member of the PDZ family of proteins that functions as a scaffold adaptor protein regulating numerous signal transduction pathways (13). Syntenin-1 interacts with Alix to regulate exosome biogenesis (12). Tumor susceptibility gene 101 (TSG101) is a fundamental component of the ESCRT complex I involved in regulating the trafficking of proteins throughout the endosomal compartment (14). TSG101 is involved in regulating diverse biological processes, such as cell proliferation, viral budding and release, and exosome biosynthesis (15,16). The heat shock protein HSP70 is a molecular chaperone involved in protein folding that can be induced upon environmental stress (17). HSP70 may also be secreted through exosomes (18).

  1. Raposo, G. and Stoorvogel, W. (2013) J Cell Biol 200, 373-83.
  2. van Niel, G. et al. (2018) Nat Rev Mol Cell Biol 19, 213-228.
  3. Jeppesen, D.K. et al. (2019) Cell 177, 428-445.e18.
  4. Kowal, J. et al. (2016) Proc Natl Acad Sci U S A 113, E968-77.
  5. Sidhom, K. et al. (2020) Int J Mol Sci 21, 6466. doi: 10.3390/ijms21186466.
  6. Patel, G.K. et al. (2019) Sci Rep 9, 5335.
  7. Tauro, B.J. et al. (2012) Methods 56, 293-304.
  8. Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
  9. de Gassart, A. et al. (2003) Blood 102, 4336-44.
  10. Katoh, K. et al. (2003) J Biol Chem 278, 39104-13.
  11. Chatellard-Causse, C. et al. (2002) J Biol Chem 277, 29108-15.
  12. Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
  13. Pradhan, A.K. et al. (2020) Cancer Metastasis Rev 39, 769-781.
  14. Katzmann, D.J. et al. (2001) Cell 106, 145-55.
  15. Garrus, J.E. et al. (2001) Cell 107, 55-65.
  16. Zhong, Q. et al. (1998) Cancer Res 58, 2699-702.
  17. Nollen, E.A. and Morimoto, R.I. (2002) J Cell Sci 115, 2809-16.
  18. Zhan, R. et al. (2009) Biochem Biophys Res Commun 387, 229-33.

Pathways

Explore pathways related to this product.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Powered by Translations.com GlobalLink OneLink SoftwarePowered By OneLink