|MED1 Antibody 51613||20 µl||
||H M R Mk||220||Rabbit|
|MED12 (D9K5J) Rabbit mAb 14360||20 µl||
||H M R Mk||240||Rabbit IgG|
|MED26 (D4B1X) Rabbit mAb 14950||20 µl||
|CDK8 (D6M3J) Rabbit mAb 17395||20 µl||
||H M Mk||53||Rabbit IgG|
|Cyclin C (E6V4Z) Rabbit mAb 68179||20 µl||
||H M||30||Rabbit IgG|
|Rpb1 NTD (D8L4Y) Rabbit mAb 14958||20 µl||
||H M R Mk||250||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Gly1813 of human MED12 protein, Pro120 of human MED26 protein, Pro455 of human CDK8 protein, Pro263 of human cyclin C protein, and Glu613 of human Rpb1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His591 of human MED1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10).
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